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Effects of Dietary beta-1,3-Glucan on Expression of Immune-related Genes of Litopenaeus vannamei Exposed to Nitrite-N

文献类型: 外文期刊

作者: Zhao, Hong-Xia 1 ; Cao, Jun-Ming 1 ; Wang, An-Li 2 ; Huang, Yan-Hua 1 ; Li, Guo-Li 1 ; Lan, Han-Bing 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

2.S China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangzhou 510631, Guangdong, Peoples R China

关键词: aquaculture;aquatic animals;aquatic organisms;binding;diets;effects;evaluation;feeding;genes;metabolism;muscles;prawns;proteinases;shrimps;stress;superoxide dismutase;weight gain;Penaeus;Decapoda;Malacostraca;Crustacea;arthropods;invertebrates;animals;aquatic animals;aquatic organisms;eukaryotes;Penaeidae;shrimps;aquatic species;proteases;superoxide

期刊名称:JOURNAL OF THE WORLD AQUACULTURE SOCIETY ( 影响因子:2.512; 五年影响因子:2.47 )

ISSN:

年卷期:

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收录情况: SCI

摘要: The effects of beta-1,3-glucan on growth and expression of immune-related genes in muscle were evaluated by a 84-d feeding trial in white shrimp, Litopenaeus vannamei, fed diets containing 0, 250, 500, and 1000 mg/kg beta-1,3-glucan, respectively. After the feeding trial, the shrimp were exposed to nitrite-N (20 mg/L) for 120 h. The transcript abundance of hemocyanin, LPS/b-glucan binding protein (LGBP), Penaeidin-3, prophenoloxidase (proPO), Serine proteinase, and superoxide dismutase (SOD) was quantified by real-time PCR. The results showed that during 84-d feeding trial, 250 mg/kg beta-1,3-glucan group showed significantly higher weight gain rate than control. There were no significant differences of survival rate between groups, but survival rate correlated inversely with dietary beta-1,3-glucan levels (r2 = 0.96). Significantly upregulated LGBP were observed in 500 mg/kg beta-1,3-glucan group compared to control. After 120 h nitrite stress, the expression level of hemocyanin, LGBP, proPO, Serine proteinase, and SOD in shrimp fed with 500 or 1000 mg/kg beta-1,3-glucan were significantly higher than those in shrimp fed with other diets. Together, in this 84-d feeding trial, 250 mg/kg beta-1,3-glucan improved growth, whereas 500 mg/kg beta-1,3-glucan preferentially improved nitrite resistance, probably through upregulating expression of immune-related genes.

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