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Immunomodulatory roles and functional analysis of pre-B lymphocyte DT40 cells with the bursal-derived BSP-II treatment

文献类型： 外文期刊

作者： Feng, Xiu-Li 1 ; Zhou, Bin 1 ; Cao, Rui-Bing 1 ; Liu, Qing-Tao; Liu, Ke 1 ; Liu, Xiao-Dong 1 ; Zhang, Yuan-Peng 1 ; Hu 1 ;

作者机构： 1.Nanjing Agr Univ, Chinas Dept Agr, Div Key Lab Anim Dis Diag & Immunol, Nanjing 210095, Jiangsu, Peoples R China

关键词： Bursal-derived BSP-II;Functional analysis;Gene microarray;Immunization experiment;Pre-B lymphocyte DT40 cell

期刊名称：PEPTIDES （ 影响因子：3.75； 五年影响因子：3.389 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The bursa of Fabricius, the acknowledged central humoral immune organ, is vital to B cell differentiation. However, the regulatory function of the bursal-derived peptide on avian B cell proliferation has not been reported. BSP-II is a recently reported bursal-derived bioactive peptide. In this paper, 75 days-old chicks were twice subcutaneously immunized with BSP-II and inactivated avian influenza virus (AIV, H 9N 2 strain). It was proved that BSP-II induced a strongly AIV-specific HI antibody production in the immunized chicks. Also, BSP-II could enhance avian pre-B lymphocyte DT40 cell viability. To investigate the global patterns of gene expression in DT40 cells after BSP-II treatment, gene microarray was carried out. It was identified that the differentially expressed genes were involved in various pathways, of which six pathways were associated with signaling transductions, including ErbB signaling, MAPK signaling, Toll-like receptor signaling, Notch signaling, mTOR signaling, and Wnt signaling. Finally, RT-qPCR was used to confirm the microarray expression data. These results indicated the molecular basis of pre-B lymphocyte viability with BSP-II treatment, which provided a potential mechanism of the bursa of Fabricius on pre-B lymphocyte viability, differentiation, and development. These results are valid for the mechanism of the bursa of Fabricius on B lymphocytes development.

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