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Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus

文献类型： 外文期刊

作者： He, Zhi 2 ; Wu, Yangsheng 2 ; Xie, Jun 3 ; Wang, Taixin 4 ; Zhang, Lihong 1 ; Zhang, Weimin 2 ;

作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Dept Biol, Guangzhou 510275, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

4.Dazhong Breeding Co Ltd, Jianyang 641400, Peoples R China

关键词： Gdf9;Immunoreactivity;Ovary;Ricefield eel Monopterus albus;Testis

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

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收录情况： SCI

摘要： Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFb) superfamily. As an oocyte-derived growth factor, GDF9 plays key roles in regulating follicle development. In the present study, we identified a gdf9 homologue from the ovary of ricefield eel, and analyzed its expression both at the mRNA and protein levels. Ricefield eel Gdf9 showed high homologies with those of other teleosts, especially perciformes fish. RT-PCR analysis revealed that ricefield eel gdf9 was expressed exclusively in the ovary and testis. The mRNA levels of gdf9 in the ovary were increased significantly at the pre-vitellogenic (PV) stage and then decreased significantly along with vitellogenesis. During the natural sex change, expression of ricefield eel gdf9 was peaked at the intersexual stages. The immunoreactivity for Gdf9 was localized exclusively in the cytoplasm of the oocytes in the ovary, particularly the oocytes at early stages, but not in the oogonia. Interestingly, strong immunoreactive signals were also detected in the degenerating oocytes in the intersexual gonad. Furthermore, the Gdf9 immunoreactivity was demonstrated for the first time to be localized in the cytoplasm of spermatogonia and spermatocytes of ricefield eel, a teleost fish. Taken together, the results of present study suggested that Gdf9 may play important roles in the folliculogenesis as well as spermatogenesis in ricefield eels.

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