您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus

文献类型： 外文期刊

作者： He, Zhi 2 ; Wu, Yangsheng 2 ; Xie, Jun 3 ; Wang, Taixin 4 ; Zhang, Lihong 1 ; Zhang, Weimin 2 ;

作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Dept Biol, Guangzhou 510275, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

4.Dazhong Breeding Co Ltd, Jianyang 641400, Peoples R China

关键词： Gdf9;Immunoreactivity;Ovary;Ricefield eel Monopterus albus;Testis

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFb) superfamily. As an oocyte-derived growth factor, GDF9 plays key roles in regulating follicle development. In the present study, we identified a gdf9 homologue from the ovary of ricefield eel, and analyzed its expression both at the mRNA and protein levels. Ricefield eel Gdf9 showed high homologies with those of other teleosts, especially perciformes fish. RT-PCR analysis revealed that ricefield eel gdf9 was expressed exclusively in the ovary and testis. The mRNA levels of gdf9 in the ovary were increased significantly at the pre-vitellogenic (PV) stage and then decreased significantly along with vitellogenesis. During the natural sex change, expression of ricefield eel gdf9 was peaked at the intersexual stages. The immunoreactivity for Gdf9 was localized exclusively in the cytoplasm of the oocytes in the ovary, particularly the oocytes at early stages, but not in the oogonia. Interestingly, strong immunoreactive signals were also detected in the degenerating oocytes in the intersexual gonad. Furthermore, the Gdf9 immunoreactivity was demonstrated for the first time to be localized in the cytoplasm of spermatogonia and spermatocytes of ricefield eel, a teleost fish. Taken together, the results of present study suggested that Gdf9 may play important roles in the folliculogenesis as well as spermatogenesis in ricefield eels.

  • 相关文献

[1]Identification and comparative profiling of ovarian and testicular microRNAs in the swimming crab Portunus trituberculatus. Liu, Ping. 2018

[2]Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes. Qiao, H.,Xiong, Y. W.,Jiang, S. F.,Fu, H. T.,Sun, S. M.,Jin, S. B.,Gong, Y. S.,Zhang, W. Y.. 2015

[3]Identification and comparative analysis of piRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus). Zhou, Yi,Zhong, Huan,Xiao, Jun,Luo, Yongju,Gan, Xi,Zhou, Yi,Yan, Jinpeng,Yan, Jinpeng,Yu, Fan.

[4]三倍体虹鳟卵巢发育中细胞凋亡与发育相关基因的表达. 柳广昊,郝其睿,黄天晴,苗畅奇,孙慧智. 2016

[5]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[6]Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis. Leng, X. Q.,Cao, H.,Leng, X. Q.,Du, H. J.,Cao, H.,Leng, X. Q.,Li, C. J..

[7]Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the ovary of red swamp crayfish Procambarus clarkii. Guan, Zheng-Bing,Liao, Xiang-Ru,Shui, Yan,Xu, Zeng-Hong,Zhou, Xin,Zhou, Xin. 2014

[8]cDNA cloning and expression of Ubc9 in the developing embryo and ovary of oriental river prawn, Macrobrachium nipponense. Zhang, Fengying,Chen, Liqiao,Wu, Ping,Zhao, Weihong,Li, Erchao,Zhang, Fengying,Qin, Jianguang.

[9]Dietary n-3 LC-PUFAs affect lipoprotein lipase (LPL) and fatty acid synthase (FAS) activities and mRNA expression during vitellogenesis and ovarian fatty acid composition of female silver pomfret (Pampus argenteus) broodstock. Peng, S.,Shi, Z.,Gao, Q.,Zhang, C.,Wang, J..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台