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Mitochondrial DNA as effective molecular markers for the genetic variation and phylogeny of the family Osteoglossidae

文献类型: 外文期刊

作者: Mu, Xi-dong 1 ; Wang, Xue-jie 1 ; Song, Hong-mei 1 ; Yang, Ye-xin 1 ; Luo, Du 1 ; Gu, Dang-en 1 ; Xu, Meng 2 ; Liu, Chao; 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Pe

关键词: Osteoglossidae;Phylogenetic analysis;Species identification

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

年卷期:

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收录情况: SCI

摘要: The present study examined the genetic variation of the family Osteoglossidae from different geographical locations based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) and ATPase subunit 6 (ATPase6) genes; we then re-constructed the phylogenetic relationships using the two sequences in combination. The results showed that the partial sequences of mitochondrial ND2 and ATPase6 of the family Osteoglossidae were 813. bp and 669. bp, respectively. A total of 42 species-specific nucleotide positions of the family Osteoglossidae were found to be useful for molecular identification. The sequence variation showed greater differences (8.3%. ~. 28.1% for the combined sequences, 8.3%. ~. 26.7% for the ND2 gene, and 9.3%. ~. 28.7% for the ATPase6 gene) among the different species of Osteoglossidae, and there was a significant association between the genetic difference and geographical location. Phylogenetic analyses using neighbor-joining, Bayesian inference, and maximum parsimony (MP) methods based on the combined sequences of the two genes were able to distinguish the different species and were in agreement with the existing taxonomy based on morphological characters and in association with the geographical distribution among seven species of the family Osteoglossidae.

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