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Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes

文献类型： 外文期刊

作者： Lin, Yaqiu 1 ; Zhang, Zhen 1 ; Tian, Yongsheng 2 ; Zhao, Wei 2 ; Zhu, Bo 2 ; Xu, Zhisheng 1 ; Peng, Rihe 2 ; Yao, Quanhon 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China

关键词： Laccase;Pichia pastoris;Coprinus cinereus;Purification;Biochemical properties;Dye decolorization

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Laccase is a blue copper oxidase with multiple copper ions and widely distributed in higher plant and fungi. To date, numerous fungal laccases have been reported by many researchers. In present work, a new laccase gene, named CcLCC5I, from Coprinus cinereus was synthesized chemically according to the yeast bias codon and integrated into Pichia pastoris GS115 genome by electroporation. SDS-PAGE analysis showed that the recombinant laccase has a molecular mass of approximately 56.8 kDa. Its biochemicalproperties was carried out using substrate 2-2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS). It was showed that the optimum pH and temperature of the laccase is 3.0 and 55 °C, respectively. Except for copper ions, most metal ions inhibited the laccase activity at a high concentration about 10 mM. Sodium sulfite can also highly inhibit laccase activity whereas EDTA had no inhibitory effect on the laccase activity. The CcLCC5I have high ability to decolor not only azo but also aryl methane dyes. The recombinant laccase decolored 44.6 % orange G, 54.8 % Crystal Violet, and 87.2 % Malachite green at about 2.6 h. The novel laccase may be a good candidate for breeding engineering strains used in the treatment of industrial effluent containing azoand aryl methane dyes.

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