您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A multiplex reverse transcription-PCR assay for the detection of influenza A virus and differentiation of the H1, H3, H5 and H9 subtypes

文献类型： 外文期刊

作者： Wu, Ling 1 ; Ding, Longfei 1 ; Pei, Zenglin 1 ; Huo, Xixiang 2 ; Wen, Guoyuan 3 ; Pan, Zishu 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

2.Hubei Prov Ctr Dis Control & Prevent, Wuhan 430079, Peoples R China

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, Peoples R China

关键词： Influenza A virus;Multiplex PCR;Subtyping

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A multiplex reverse transcription-PCR (mRT-PCR) assay was developed for the rapid detection of influenza A viruses. The assay simultaneously differentiated H1, H3, H5 and H9 hemagglutinin subtypes in a single reaction mixture. Five sets of specific primers targeted to the M, H1, H3, H5 and H9 genes were used in this assay. The amplified products were visualized by agarose gel electrophoresis. The sizes of the PCR amplified fragments were 612 bp for H1, 187 bp for H3, 338 bp for H5, 289 bp for H9 and 239 bp for M. The detection limit of the viral RNA template was 1 ng for the H1, H3 and H5 subtypes and 0.1 ng for the H9 subtype. Nonspecific product bands from RNAs of other viral pathogens were not amplified. The sensitivity analysis demonstrated that the mRT-PCR assay is as sensitive as conventional RT-PCR and 10 times less sensitive than SYBR Green real-time RT-PCR. In conclusion, the mRT-PCR assay developed in this study was able to type influenza A viruses and simultaneously differentiate H1, H3, H5 and H9 subtypes in both human and avian clinical specimens, and thus, the mRT-PCR assay could be a rapid, convenient and relatively inexpensive molecular diagnostic tool for large-scale screening of clinical samples

  • 相关文献

[1]Simultaneous identification and combined genotype effects analysis of pre-implantation protein 3 (prei3) and estrogen receptor (ESR) gene in pigs. Li, F. E.,Ye, L. Z.,Niu, B. Y.,Luo, L. F.,Shao, G. C.,Deng, C. Y.,Jiang, S. W.,Xiong, Y. Z.,Li, F. E.,Niu, B. Y.,Shao, G. C..

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台