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Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis

文献类型： 外文期刊

作者： Xiong, Ai-Sheng 1 ; Jiang, Hai-Hua 2 ; Zhuang, Jing 3 ; Peng, Ri-He 2 ; Jin, Xiao-Fen 2 ; Zhu, Bo 2 ; Wang, Feng 1 ; Zhan 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China

3.Alberta Innovates Technol Futures Alberta Res Cou, Vegreville, AB T9C 1T4, Canada

关键词： AP2/ERF;Arabidopsis;Brassica napus;Cold stress;Directed evolution;Transcription factor

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

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收录情况： SCI

摘要： One of the most rapid and effective defensive mechanisms plants have for protecting themselves, from a variety of biotic and abiotic stresses, is the regulation of plant signal transcription factors. AP2/ERF factors play an important role in plant development as well as in hormonal regulation and cold response. Directed evolution is a powerful tool to modify proteins, improving their properties, and for studying their structure-function relations. Here, the transgenic Arabidopsis plants over-expressed a mutant gene, BnaERF-B3-hy15-mu3, which encoded for a factor that exhibited more binding activity with the GCC box element than the wild-type gene BnaERF-B3-hy15 encode factor, and exhibited more freezing tolerance than transgenic plants containing the original BnaERF-B3-hy15 gene. Real-time PCR analyses also revealed that the expression levels of several stress-regulated genes were altered in the over-expressed BnaERF-B3-hy15-mu3 transgenic lines. The BnaERF-B3-hy15 responded to exogenous ABA. Using RT-PCR analysis, the expression of BnaERF-B3-hy15 at different stages and stress treatments were also analyzed.

  • 相关文献

[1]Transcription factor OsAP21 gene increases salt/drought tolerance in transgenic Arabidopsis thaliana. Jin, Xiaofeng,Xue, Yong,Xu, RanRan,Bian, Lin,Zhu, Bo,Han, Hongjuan,Peng, Rihe,Yao, Quanhong,Wang, Ren.

[2]Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Zhuang, Jing,Yao, Quan-Hong,Sun, Chao-Cai,Zhou, Xi-Rong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min,Xiong, Fei.

[3]Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution. Jin, Xiao-Fen,Zhu, Bo,Peng, Ri-He,Jiang, Hai-hua,Yao, Quan-Hong,Xiong, Ai-Sheng,Jiang, Hai-hua,Chen, Jian-Min,Zhuang, Jing,Zhang, Jian.

[4]Discovery, phylogeny and expression patterns of AP2-like genes in maize. Zhuang, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Deng, De-Xiang,Xiong, Fei,Chen, Jian-Min,Zhuang, Jing,Zhang, Jian.

[5]Analysis of Brassica rapa ESTs: gene discovery and expression patterns of AP2/ERF family genes. Zhuang, Jing,Xiong, Ai-Sheng,Peng, Ri-He,Gao, Feng,Zhu, Bo,Fu, Xiao-Yan,Jin, Xiao-Feng,Yao, Quan-Hong,Zhang, Zhen,Qiao, Yu-Shan,Zhuang, Jing,Zhang, Jian,Chen, Jian-Min.

[6]Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.. Liu, Jin-Ge,Zhang, Zhen,Qin, Qiu-Lin,Peng, Ri-He,Xiong, Ai-Sheng,Chen, Jian-Min,Xu, Fang,Zhu, Hong,Yao, Quan-Hong.

[7]A novel Cys(2)/His(2) zinc finger protein gene from sweetpotato, IbZFP1, is involved in salt and drought tolerance in transgenic Arabidopsis. Wang, Feibing,Peng, Rihe,Yao, Quanhong,Zhu, Hong,Liu, Qingchang,Tong, Wenjie,Kong, Weili.

[8]甘蓝型油菜中一类AP2/ERF转录因子的克隆和生物信息学分析. 庄静,周熙荣,孙超才,管帮超,彭日荷,乔玉山,章镇,高峰,蔡斌,熊爱生,姚泉洪. 2008

[9]沪油15中两个AP2/ERF-B1亚族转录因子的克隆与分析. 庄静,彭日荷,高峰,付晓燕,朱波. 2009

[10]Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Li, Xian,Xue, Yong,Liu, Jin-Ge,Gao, Feng,Cai, Bin,Chen, Jian-Min,Yao, Quan-Hong.

[11]Directed evolution of beta-galactosidase from Escherichia coli into beta-glucuronidase. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Xu, Fang,Cai, Bin,Guo, Zhao-Kui,Qiao, Yu-Shan,Chen, Jian-Min,Zhang, Zhen,Yao, Quan-Hong. 2007

[12]Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability. Xiong, Ai-Sheng,Peng, Ri-He,Cheng, Zong-Ming,Li, Yi,Liu, Jin-Ge,Zhuang, Jing,Gao, Feng,Xu, Fang,Qiao, Yu-Shan,Zhang, Zhen,Chen, Jian-Min,Yao, Quan-Hong. 2007

[13]A semi-rational design strategy of directed evolution combined with chemical synthesis of DNA sequences. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Gao, Feng,Xu, Fang,Cai, Bin,Yao, Quan-Hong.

[14]A Rice OsAP23, Functioning as an AP2/ERF Transcription Factor, Reduces Salt Tolerance in Transgenic Arabidopsis. Zhuang, Jing,Wang, Feng,Xiong, Ai-Sheng,Jiang, Hai-Hua,Peng, Ri-He,Yao, Quan-Hong.

[15]Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa. Zhuang, Jing,Cai, Bin,Peng, Ri-He,Zhu, Bo,Jin, Xiao-Feng,Xue, Yong,Gao, Feng,Fu, Xiao-Yan,Tian, Yong-Sheng,Zhao, Wei,Xiong, Ai-Sheng,Yao, Quan-Hong,Zhuang, Jing,Cai, Bin,Qiao, Yu-Shan,Zhang, Zhen. 2008

[16]Melatonin alleviates cold-induced oxidative damage by regulation of ascorbate-glutathione and proline metabolism in melon seedlings (Cucumis melo L.). Zhang, Y. P.,Chen, Y. Y.,Zhang, Y. P.,Xu, S.,Yang, S. J.,Chen, Y. Y..

[17]Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis. Xue, Yong,Peng, Rihe,Xiong, Aisheng,Li, Xian,Yao, Quanhong,Zha, Dingshi.

[18]Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza sativa L.. Fu, Xiao-Yan,Zhang, Zhen,Peng, Ri-He,Xiong, Ai-Sheng,Liu, Jin-Ge,Wu, Li-Juan,Gao, Feng,Zhu, Hong,Guo, Zhao-Kui,Yao, Quan-Hong. 2007

[19]Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15. Zhuang, Jing,Sun, Chao-Cai,Zhou, Xi-Rong,Zhuang, Jing,Zhang, Jian,Xiong, Ai-Sheng. 2011

[20]Genome-Wide Identification and Analysis of the MYB Transcription Factor Superfamily in Solanum lycopersicum. Li, Zhenjun,Peng, Rihe,Tian, Yongsheng,Han, Hongjuan,Xu, Jing,Yao, Quanhong.

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