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Gloverins of the silkworm Bombyx mori: Structural and binding properties and activities

文献类型： 外文期刊

作者： Yi, Hui-Yu 1 ; Deng, Xiao-Juan 1 ; Yang, Wan-Ying 1 ; Zhou, Cong-Zhao 3 ; Cao, Yang 1 ; Yu, Xiao-Qiang 2 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Lab Insect Mol Biol & Biotechnol, Guangzhou 510642, Guangdong, Peoples R China

2.Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA

3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

关键词： Gloverin;Lipopolysaccharide;Circular dichroism;Antibacterial;Random coil;about-helix;Bombyx mori

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

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收录情况： SCI

摘要： Gloverins are basic, glycine-rich and heat-stable antibacterial proteins (~ 14- kDa) in lepidopteran insects with activity against Escherichia coli, Gram-positive bacteria, fungi and a virus. Hyalophora gloveri gloverin adopts a random coil structurein aqueous solution but has a-helical structure in membrane-like environment, and it may interact with the lipid A moiety of lipopolysaccharide (LPS). Manduca sexta gloverin binds to the O-specific antigen and outer core carbohydrate of LPS. In the silkworm Bombyx mori, there are four gloverins with slightly acidic to neutral isoelectric points. In this study, we investigate structural and binding properties and activities of. mori gloverins (BmGlvs), as well as correlations between structure, binding property and activity. Recombinant mGlvl -4 were expressed in bacteria and purified. Circular dichroism (CD) spectra showed that all four BmGlvs mainly adopted random coli structure (>50%) in aqueous solution in regardless of pH, but contained a-helical structure in the presence of l,l,l,3,3,3-hexafluoro-2-propanol (HFIP), smooth and rough mutants (Ra, Rc and Re) of LPS and lipid A. Plate ELISA assay showed that BmGlvs at pH 5.0 bound to rough mutants of LPS and lipid A but not to smooth LPS. Antibacterial activity assay showed that positively charged BmGlvs (at pH 5.0) were active against E. coli mutant strains containing rough LPS but inactive against E. coli with smooth LPS. Our results suggest that binding to rough LPS is the prerequisite for the activity of BmGlvs against E. coli.

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