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An Improved Integrative Transformation System for Pichia pastoris with DNA-polyethylenimine-dextran Sulfate Nanoparticles

文献类型： 外文期刊

作者： Xue, Wei 1 ; Wang, Hang 1 ; Pei, Zuodi 1 ; Zhao, Wei 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotechnol Res Inst, Shanghai 201106, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Shanghai Ocea

关键词： DNA nanoparticles;integration efficiency;polyethyleneimine;yeast electroporation

期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it difficult to obtain a large number of transformants necessary to thoroughly cover a large library. We report here that pre-coating DNA with polyethyleneimine and dextran sulfate increased gene integration significantly in Pichia electroporation. This improvement on integrative efficiency has been proved in different voltage, resistance, cell phase, and DNA concentrations. The condensed DNA nanoparticles make Pichia available as a host for screening large libraries of random mutants.

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