An Improved Integrative Transformation System for Pichia pastoris with DNA-polyethylenimine-dextran Sulfate Nanoparticles
文献类型： 外文期刊
作者： Xue, Wei 1 ; Wang, Hang 1 ; Pei, Zuodi 1 ; Zhao, Wei 1 ; Tian, Yong-Sheng 1 ; Peng, Ri-He 1 ; Yao, Quan-Hong 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotechnol Res Inst, Shanghai 201106, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Shanghai Ocea
关键词： DNA nanoparticles;integration efficiency;polyethyleneimine;yeast electroporation
期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it difficult to obtain a large number of transformants necessary to thoroughly cover a large library. We report here that pre-coating DNA with polyethyleneimine and dextran sulfate increased gene integration significantly in Pichia electroporation. This improvement on integrative efficiency has been proved in different voltage, resistance, cell phase, and DNA concentrations. The condensed DNA nanoparticles make Pichia available as a host for screening large libraries of random mutants.
- 相关文献
作者其他论文 更多>>
-
The complete degradation of 1,2-dichloroethane in Escherichia coli by metabolic engineering
作者：Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong;Deng, Yong-Dong;Zhang, Wen -Hui;Zuo, Zhi-Hao;Zhang, Hao;Xu, Jing;Gao, Jian- Jie;Wang, Bo;Li, Zhen-Jun;Fu, Xiao-Yan;Wang, Li -Juan;Wang, Yu;Tian, Yong-Sheng;Peng, Ri-He;Yao, Quan-Hong
关键词：Bioremediation; Engineered bacteria; Environmental pollution; Metabolic engineering; Synthetic biology
-
"Structure-function" analysis using starches isolated from Lycoris chinensis bulbs of different developmental stages
作者：Ren, Huanhuan;Zhao, Wei;Li, Kehu;Zhang, Tongze;Li, Qingzhu;He, Liangliang
关键词：Lycoris chinensis; Starch physicochemical properties; Structural properties
-
Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation
作者：Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Zhang, Wen-Hui;Deng, Yong-Dong;Zuo, Zhi-Hao;Tian, Yong-Sheng;Xu, Jing;Wang, Bo;Wang, Li-Juan;Han, Hong-Juan;Li, Zhen-Jun;Wang, Yu;Yao, Quan-Hong;Gao, Jian-Jie;Fu, Xiao-Yan;Peng, Ri-He;Chen, Zhi-Feng
关键词：4-Dinitrotoluene; Re-synthesize gene; Complete degradation; Bioremediation; Escherichia coli
-
Creation of Environmentally Friendly Super "Dinitrotoluene Scavenger" Plants
作者：Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Xu, Jing;Wang, Bo;Fu, Xiao-Yan;Han, Hong-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Wang, Yu;Zuo, Zhi-Hao;Peng, Ri-He;Tian, Yong-Sheng;Yao, Quan-Hong;Zhu, Bo
关键词：2,4-dinitrotoluene (2,4-DNT); carbon neutrality; complete degradation; rapid phytoremediation; sustainable development goals
-
Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
作者：Wang, Bo;Wang, Ya-Hui;Deng, Yuan-Jie;Xiong, Ai-Sheng;Wang, Bo;Yao, Quan-Hong
关键词：Genetically modified carrot; Betanin; Unintended effects; Photosynthesis; Transcriptome; Metabolomics
-
Complete biodegradation of the oldest organic herbicide 2,4-Dichlorophe- noxyacetic acid by engineering Escherichia coli
作者：Wang, Yu;Tian, Yong-Sheng;Gao, Jian-Jie;Xu, Jing;Li, Zhen-Jun;Fu, Xiao-Yan;Han, Hong-Juan;Wang, Li-Juan;Zhang, Wen-Hui;Deng, Yong-Dong;Qian, Cen;Zuo, Zhi-Hao;Wang, Bo;Peng, Ri-He;Yao, Quan-Hong
关键词：24-Dichlorophenoxyacetic acid; Biodegradation; Environmental pollution; Engineered bacteria; Synthetic biology
-
Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff
作者：Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong;Peng, Ri-He;Zhang, Wen-Hui;Wang, Yu;Deng, Yong-Dong;Wang, Bo;Gao, Jian-Jie;Li, Zhen-Jun;Wang, Li-Juan;Fu, Xiao-Yan;Xu, Jing;Han, Hong-Juan;Tian, Yong-Sheng;Yao, Quan-Hong
关键词：recombinant enzymes; feed additive; feedstuff; nutrient utilization
