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Global Aspects of pacC Regulation of Pathogenicity Genes in Colletotrichum gloeosporioides as Revealed by Transcriptome Analysis

文献类型： 外文期刊

作者： Alkan, Noam 1 ; Meng, Xiangchun 2 ; Friedlander, Gilgi 1 ; Reuveni, Eli 1 ; Sukno, Serenella 4 ; Sherman, Amir 5 ; Tho 1 ;

作者机构： 1.Weizmann Inst Sci, Dept Plant Sci, IL-76100 Rehovot, Israel

2.Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-50250 Bet Dagan, Israel

3.Guangdong Acad Agr Sci, Fruit Tree Res Inst, Guangzhou 510640, Guangdong, Peoples R China

4.Univ Salamanca, Dept Genet & Microbiol, Ctr Hispanoluso Invest Agr CIALE, Villamayor 37185, Spain

5.Agr Res Org, Volcani Ctr, Inst Plant Sci, Genom Unit, IL-50250 Bet Dagan, Israel

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

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收录情况： SCI

摘要： Colletotrichum gloeosporioides alkalinizes its surroundings during colonization of host tissue. The transcription factor pacC is a regulator of pH-controlled genes and is essential for successful colonization. We present here the sequence assembly of the Colletotrichum fruit pathogen and use it to explore the global regulation of pathogenicity by ambient pH. The assembled genome size was 54 Mb, encoding 18,456 genes. Transcriptomes of the wild type and Delta pacC mutant were established by RNA-seq and explored for their global pH-dependent gene regulation. The analysis showed that pacC upregulates 478 genes and downregulates 483 genes, comprising 5% of the fungal genome, including transporters, antioxidants, and cell-wall-degrading enzymes. Interestingly, gene families with similar functionality are both up- and downregulated by pacC. Global analysis of secreted genes showed significant pacC activation of degradative enzymes at alkaline pH and during fruit infection. Select genes from alkalizing-type pathogen C. gloeosporioides and from acidifying-type pathogen Sclerotinia sclerotiorum were verified by quantitative reverse-transcription polymerase chain reaction analysis at different pH values. Knock out of several pacC-activated genes confirmed their involvement in pathogenic colonization of alkalinized surroundings. The results suggest a global regulation by pacC of key pathogenicity genes during pH change in alkalinizing and acidifying pathogens.

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