Expression of PEI-coated gold nanoparticles carrying exogenous gene in periwinkle mesophyll cells and its practice in huanglongbing research
文献类型： 外文期刊
作者： Zhang, Junzhe 1 ; Lei, Wenxue 1 ; Meng, Yixuan 1 ; Zhou, Chengqian 4 ; Zhang, Baihong 1 ; Yuan, Jun 5 ; Wang, Min 5 ; Xu, Dongqing 6 ; Meng, Xiangchun 3 ; Chen, Wenli 1 ;
作者机构： 1.South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangdong Prov Key Lab Laser Life Sci,Guangzhou K, Guangzhou 510631, Peoples R China
2.South China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Peoples R China
3.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Key Lab South Subtrop Fruit Biol & Genet Resource, Key Lab Trop & Subtrop Fruit Tree Res, Guangzhou 510640, Peoples R China
4.Hugo Moser Res Inst Kennedy Krieger, Neurosci Lab, Baltimore, MD 21205 USA
5.Nanjing Agr Univ, Coll Resources & Environm Sci, Weigang 1, Nanjing 210095, Peoples R China
6.Nanjing Agr Univ, Coll Life Sci, Weigang 1, Nanjing 210095, Peoples R China
期刊名称：ISCIENCE （ 影响因子：6.107； 五年影响因子：6.233 ）
ISSN：
年卷期： 2022 年 25 卷 6 期
页码：
收录情况： SCI
摘要： Huanglongbing (HLB) is a devastating disease of citrus, which is mostly caused by Candidatus Liberibacter asiaticus (CLas). To realize the specific application of nano-transgenic technology in HLB, AuNPs-PEI (Gold Nanoparticles-Polyethylenimine) was used to carry foreign genes into the leaves of periwinkle (Catharanthus roseus) by infiltration. Here, we demonstrated that NPR1-GFP protein expression was observed from the 12th hour to the 10th day after infiltrating AuNPs-PEI- pNPR1 (Arabidopsis thaliana nonexpressor of pathogenesis-related gene 1)-GFP. Fluorescence of mCherry was observed 6 h after AuNPs-PEI-pNLS (nuclear localization signal sequence)-mCherry infiltration and fluorescence of FAM was observed in the nucleus 4 h after AuNPs-PEI-FAM-siRNA(NPR1) infiltration. In addition, NPR1-GFP expression in CLas-infected periwinkle leaves was significantly higher than that in healthy periwinkle leaves after infiltration. Our work confirmed that the expression of exogenous NPR1-GFP could reduce the CLas titersby promoting the expression of PR (pathogenesis related) genes and ICS (isochorismate synthase) gene.
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