Identification of two combined genes responsible for dechlorination of 3,5,6-trichloro-2-pyridinol (TCP) in Cupriavidus pauculus P2
文献类型： 外文期刊
作者： Cao, Li 1 ; Xu, Jianhong 2 ; Wu, Guang 1 ; Li, Mingxing 1 ; Jiang, Jiandong 1 ; He, Jian 1 ; Li, Shunpeng 1 ; Hong, Qing 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr, Key Lab Agr Environm Microbiol, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Ctr GMO Evaluat & Detect, Minist Agr, Key Lab Agroprod Safety Risk Evaluat Nanjing, Nanjing 210014, Jiangsu, Peoples R China
关键词： Cupriavidus pauculus P2;3;5;6-Trichloro-2-pyridinol;Gene cluster;Dechlorination
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：10.588； 五年影响因子：10.129 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Dehalogenation is an important mechanism for degrading and detoxifying halogenated aromatics in microbes. However, the biochemical and molecular mechanisms of dehalogenation of 3,5,6-trichloro-2-pyridinol (TCP) are still unknown. In this study, a novel 6012 bp gene cluster was cloned from TCP-degrading strain P2, which was responsible for the dehalogenation of TCP. The cluster included a monooxygenase gene (tcpA1), a flavin reductase gene (tcpB1), tcpR1, orf1 and orf2. TcpA1 and TcpB1 were indispensable for the dehalogenation of TCP. They worked together to catalyze the dehalogenation of three chlorine of TCP, and generated a more readily biodegradable product of 3,6-dihydroxypyridine-2,5-dione. TcpA1 displayed the highest activity against TCP at 40℃ and at pH 8.0. Cu~(2+), Zn~(2+), and Hg~(2+) significantly inhibited enzyme activity. To the best of our knowledge, this is the first report on a gene cluster responsible for TCP degradation.
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