Identification of duck HSP70 gene, polymorphism analysis and tissue expression under control and heat stress conditions
文献类型： 外文期刊
作者： Xia, M. 1 ; Gan, J. 2 ; Luo, Q. 2 ; Zhang, X. 2 ; Yang, G. 2 ;
作者机构： 1.Univ Nebraska, Dept Nutr & Hlth Sci, Coll Educ & Human Sci, Lincoln, NE USA
2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
3.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China
4.South China Agr Univ, Key Lab Chicken Genet Breeding & Repro
期刊名称：BRITISH POULTRY SCIENCE （ 影响因子：2.095； 五年影响因子：2.266 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： 1. This study was designed to characterise the duck heat shock protein 70 gene (HSP70) and identify sequence variation.2. Chicken HSP70 sequence (GenBank: AY143693) was used as a template to design a primer pair to amplify partial duck HSP70 gene. Primers were subsequently designed with the duck HSP70 gene as a template to amplify the complete duck HSP70 sequence.3. Twelve commercial Sanshui White ducklings were subjected to a heat stress experiment. Tissue samples were collected for RNA extraction and real-time PCR to analyse the expression mechanism of duck HSP70.4. A DNA pool was constructed from three different species for single nucleotide polymorphism (SNP) screening. The genotypes of the identified SNPs were detected in 145 Sanshui White ducklings.5. Duck HSP70 gene was identified and characterised (GenBank: EU678246) and shown to contain no introns. Fifteen variations were identified within the open reading frame. Quantitative real-time PCR results showed that the expression of duck HSP70 gene was tissue specific and the highest expression level was seen in pectoral muscle.
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