Mouse undifferentiated spermatogonial stem cells cultured as aggregates under simulated microgravity
文献类型： 外文期刊
作者： Zhang, X. 1 ; Li, L. 1 ; Bai, Y. 1 ; Shi, R. 1 ; Wei, H. 1 ; Zhang, S. 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Guangdong Prov key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
2.South China Inst Endangered Anim, Guangdong Entomol Inst, Guangzhou, Guangdong, Peoples R China
关键词： Fibrin scaffolds;mouse spermatogonial stem cells;simulated microgravity
期刊名称：ANDROLOGIA （ 影响因子：2.775； 五年影响因子：2.638 ）
ISSN： 0303-4569
年卷期： 2014 年 46 卷 9 期
页码：
收录情况： SCI
摘要： Dynamic simulated microgravity (SMG) culture systems provide environments that stimulate stem cell proliferation and differentiation. However, the effect of SMG on spermatogonial stem cells (SSCs) remains unclear. Here, we used a rotating cell culture system (RCCS) to determine its effect on mouse SSC proliferation and differentiation. SSCs were enriched from mouse pub testis and cocultured with Sertoli cell feeders pre-treated with mitomycin C on fibrin scaffolds in a rotary bioreactor for 14 days. Our results show that mouse SSCs cultured in a rotary bioreactor exhibited enhanced proliferation surpassing those cultured in static conditions, although SSC cultures in SMG underwent a growth lag at initial 3 days. After 14 days, mouse SSCs and feeders grew into cell aggregates with average diameters of 242.63 +/- 16.53 mu m compared with those in conventional static culture (49.51 +/- 15.64 mu m). Related detection revealed that proliferating SSCs in SMG remained undifferentiated, maintained clone-forming capacity and were capable of differentiation into round spermatids with flagella. The growth characteristics of mouse SSCs in RCCS suggest that the resulting aggregates are similar to native in vivo cells. Rotary bioreactors that create SMG environments may be an alternative to conventional systems for the clinical application of SSCs.
- 相关文献
作者其他论文 更多>>
-
Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens
作者：Ruan, D.;Fan, Q. L.;Zhang, S.;Lin, X. J.;Zheng, C. T.;Jiang, Z. Y.;Jiang, S. Q.;EI-Senousey, H. K.;Fouadt, A. M.;Yan, S. J.;Yan, S. J.
关键词：isoleucine; yellow -feathered chickens; amino acid transporters; protein metabolism; gut microbiota
-
Protective effects of protocatechuic acid on growth performance, intestinal barrier and antioxidant capacity in broilers challenged with lipopolysaccharide
作者：Jiang, S. Q.;Chen, Z. L.;Zhang, S.;Ye, J. L.;Wang, Y. B.
关键词：Antioxidant effect; Feed additive; Intestinal morphology; Lipopolysaccharide challenge; Yellow feather broiler
-
Modelling nitrogen-corrected apparent metabolisable energy requirement for egg production of 3 BW types of Yellow Broiler breeder hens during the egg-laying period
作者：Gou, Z.;Jiang, Z.;Zhang, S.;Lin, X.;Wang, Y.;Ruan, D.;Fan, Q.;Ye, J.;Mao, S.;Jiang, S.
关键词：Factorial approach; Maintenance; Gompertz; Body weight; Validation
-
Physiological effects of transport duration on stress biomarkers and meat quality of medium-growing Yellow broiler chickens
作者：Gou, Z.;Abouelezz, K. F. M.;Fan, Q.;Li, L.;Lin, X.;Wang, Y.;Cui, X.;Ye, J.;Jiang, S.;Ma, X.;Abouelezz, K. F. M.;Masoud, M. A.
关键词：BW loss; Indigenous chickens; Meat analysis; Plasma metabolites; Pre-slaughter handling
-
EFFECTS OF TRANSPLANTING CONDITIONS AND WATER MANAGEMENT ON THE YIELD, QUALITY AND AROMA OF FRAGRANT RICE
作者：Tong, T. Y.;Zhang, J. S.;Li, L.;Luo, H. W.;Cai, J. X.;Ma, L.;Mo, Z. W.;Tang, X. R.;Tong, T. Y.;Zhang, J. S.;Li, L.;Luo, H. W.;Ma, L.;Mo, Z. W.;Tang, X. R.;He, R. J.
关键词：rice; rainfed; aroma-related enzymes; 2-acetyl-1-pyrroline; yield
-
Effects and interaction of dietary calcium and non-phytate phosphorus for slow-growing yellow-feathered broilers during the starter phase
作者：Wang, Y. B.;Wang, W. W.;Fan, Q. L.;Ye, J. L.;Zhang, S.;Jiang, S. Q.;Wang, W. W.
关键词：Alkaline phosphatase; Bone; Dietary nutrient levels; Performance; Tibial characteristics
-
Alterations of the fatty acid composition and lipid metabolome of breast muscle in chickens exposed to dietary mixed edible oils
作者：Cui, X. Y.;Gou, Z. Y.;Abouelezz, K. F. M.;Li, L.;Lin, X. J.;Fan, Q. L.;Wang, Y. B.;Cheng, Z. G.;Ding, F. Y.;Jiang, S. Q.;Abouelezz, K. F. M.
关键词：Qingyuan chickens; coconut oil; growth performance; n-3 fatty acids; phospholipids
