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20-hydroxyecdysone upregulates Atg genes to induce autophagy in the Bombyx fat body

文献类型： 外文期刊

作者： Tian, Ling 1 ; Ma, Li 1 ; Guo, Enen 1 ; Deng, Xiaojuan 4 ; Ma, Sanyuan 2 ; Xia, Qingyou 2 ; Cao, Yang; Li, Sheng 1 ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Insect Dev & Evolutionary Biol, Shanghai, Peoples R China

2.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing, Peoples R China

3.South China Agr Univ, Coll Anim Sci, Lab Insect Mol Biol & Biotechnol, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China

4.South China Agr Univ, Coll Anim Sci, Lab Insect Mol Biol & Biotechnol, Guangdon

关键词： 2Q-hydroxyecdysone;fat body;autophagy;Atg genes;Atgl;ATG8;transcriptional regulation;TORC1;Bombyx mori

期刊名称：AUTOPHAGY （ 影响因子：16.016； 五年影响因子：16.586 ）

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收录情况： SCI

摘要： Autophagy is finely regulated at multiple levels and plays crucial roles in development and disease. In the fat body of the silkworm, Bombyx mori, autophagy occurs and Atg gene expression peaks during the nonfeeding molting and pupation stages when the steroid hormone (20-hydroxyecdysone; 20E) is high. Injection of 20E into the feeding larvae upregulated Atg genes and reduced TORC1 activity resulting in autophagy induction in the fat body. Conversely, RNAi knockdown of the 20E receptor partner (USP) or targeted overexpression of a dominant negative mutant of the 20E receptor (EcR~(DN)) in the larval fat body reduced autophagy and downregulated the Atg genes, confirming the importance of 20E-induction of Atg gene expression during pupation. Moreover, in vitro treatments of the larval fat body with 20E upregulated the Atg genes. Five Atg genes were potentially 20E primary-responsive, and a 20E response element was identified in the Atg1 (ortholog of human ULK1) promoter region. Furthermore, RNAi knockdown of 4 key genes (namely Br-C, E74, HR3 and betaftz-F1) in the 20E-triggered transcriptional cascade reduced autophagy and downregulated Atg genes to different levels. Taken together, we conclude that in addition to blocking TORC1 activity for autophagosome initiation, 20E upregulates Atg genes to induce autophagy in the Bombyx fat body.

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