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Chemical analysis and antioxidant activity of polysaccharides extracted from Inonotus obliquus sclerotia

文献类型： 外文期刊

作者： Du, XiuJu 1 ; Mu, HongMei 2 ; Zhou, Shuai 3 ; Zhang, Yang 1 ; Zhu, XiaoLi 1 ;

作者机构： 1.Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China

2.Liaocheng Univ, Coll Agr Sci, Liaocheng 252059, Shandong, Peoples R China

3.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China

关键词： Inonotus obliquus polysaccharide;Free radicals scavenging activity;Monosaccharide composition;Antioxidant activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN：

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收录情况： SCI

摘要： Three water-soluble polysaccharide fractions (IOP40, IOP60 and IOP80) were isolated by using different concentrations of alcohol precipitation from Inonotus obliquus sclerotia. Their physicochemical properties, including total sugar content, protein content, monosaccharide composition and percentage were analyzed. And their in vitro antioxidant capacities were investigated in terms of reducing power assay and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, hydroxyl radicals, superoxide anion radicals and hydrogen peroxide (H_2O_2). In general, three polysaccharide fractions exhibited increasing antioxidant activity with increasing concentration at the ranges of tested dosage. The orders of reducing power, DPPH-scavenging capacity, H_2O_2-scavenging capacity, and hydroxyl-scavenging activity were all IOP60 > IOP40 > IOP80. These findings demonstrated that three polysaccharide fractions extracted from I. obliquus, especially IOP60, could be employed as natural ingredients in functional food and pharmaceutical industry to alleviate the oxidative stress.

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