Mutation Breeding of Lipase-producing Strain Flavobacterium sp by Supercritical CO2 with Hydrazine Hydrate
文献类型： 外文期刊
作者： Zhang, Qiaoyan 1 ; Qian, Junqing 1 ; Ma, Lingzhi 1 ;
作者机构： 1.Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Supercritical CO2;Mutation;pH Drop;Lipase;Flavobacterium sp
期刊名称：BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY （ 影响因子：0.797； 五年影响因子：1.193 ）
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收录情况： SCI
摘要： This work aimed to obtain an ideal mutant strain with higher lipase yield using hydrazine hydrate (HZH) as a novel additive to treat Flavobacterium sp. strain YY25 by supercritical CO2. The survival rate and the positive mutation rate of the tested strain were strongly dependent on the dose of HZH. The treatment by 0.5% HZH in supercritical CO2 (8 MPa, 35 degrees C) for 30 min provided 58.3% of positive mutation rate and an expected mutant strain with about 76.7% increase in lipase yield compared with the wild strain. Possible mutagenesis mechanisms were further explored. The analysis on pH drop of the treated seed liquid was performed to better understand the interaction. Results proved that the induced mutation with enhanced yield of lipase could be achieved by co-mutagenesis of supercritical CO2 and HZH.
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