您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation

文献类型： 外文期刊

作者： Xiong, Qiyan 2 ; Wei, Yanna 2 ; Xie, Haidong 2 ; Feng, Zhixin 2 ; Gan, Yuan 3 ; Wang, Chunlai 1 ; Liu, Maojun; Bai, Fa 1 ;

作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Div Bacterial Dis, Harbin 150001, Peoples R China

2.Jiangsu Acad Agr Sci, Natl Ctr Engn Res Vet Bioprod, Key Lab Vet Biol Engn & Technol, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Natl Ctr Engn Res

关键词： Adjuvant;Intramuscular immunization;Live vaccine;Mycoplasma hyopneumoniae

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mycoplasma hyopneumoniae (M. hyopneumoniae) vaccine strain 168 is an intrapulmonically injected attenuated live vaccine that is available in the Chinese market. The aim of this study was to develop suitable adjuvants for this live vaccine to provide effective protection after intramuscular inoculation. Several adjuvant components were screened to assess their toxicity for the live vaccine, and various adjuvant formulations were then designed and prepared. Vaccines supplemented with these adjuvants were used to immunize mice intramuscularly to assess the capacity of the adjuvants to induce a specific immune response. The screened formulations were then evaluated in pigs. Seven of the eight adjuvant components did not affect the viability of the live vaccine, and seven different adjuvant formulations were then designed. In mice, the ISCOM-matrix adjuvant and the levamisole-chitosan mixture adjuvant significantly enhanced serum IgG responses against M. hyopneumoniae, while lymphocyte proliferation was enhanced by the ISCOM-matrix adjuvant, the carbomer-astragalus polysaccharide mixture adjuvant and an oil-in-water emulsion adjuvant. These four adjuvants were evaluated in pigs. Enhancement of specific lymphocyte proliferation responses was observed in the groups vaccinated with the ISCOM-matrix adjuvant and the carbomer-astragalus polysaccharide mixture adjuvant. Significant enhancement of serum IgG antibody production was observed before challenge in pigs vaccinated with the carbomer-astragalus polysaccharide mixture adjuvant and the levamisole-chitosan mixture adjuvant, while after challenge, all of the animals that received vaccines containing adjuvants had higher antibody concentrations against M. hyopneumoniae than unvaccinated animals. Animals inoculated with a vaccine containing the ISCOM-matrix adjuvant (median score 3.57) or the carbomer-astragalus polysaccharide mixture adjuvant (median score 528) had reduced lesion scores compared to unvaccinated animals (median score 14.81). These studies will help in the development of appropriate adjuvants for intramuscular administration of this live M. hyopneumoniae vaccine.

  • 相关文献

[1]Protective efficacy of a live attenuated Mycoplasma hyopneumoniae vaccine with an ISCOM-matrix adjuvant in pigs. Xiong, Qiyan,Wei, Yanna,Feng, Zhixin,Gan, Yuan,Liu, Zhanjun,Liu, Maojun,Bai, Fangfang,Shao, Guoqing.

[2]The swine CD81 enhances E2-based DNA vaccination against classical swine fever. Li, Wenliang,Zhou, Bin,Liu, Xia,Yang, Leilei,Zhang, Wenwen,Jiang, Jieyuan,Zhou, Bin.

[3]Salidroside liposome formulation enhances the activity of dendritic cells and immune responses. Zhao, Xiaojuan,Tao, Yang,Huang, Yee,Wang, Deyun,Hu, Yuanliang,Liu, Jiaguo,Wu, Yi,Yu, Yun,Liu, Cui,Zhao, Xiaojuan,Lu, Yu. 2013

[4]Antigen-Sparing and Enhanced Efficacy of Multivalent Vaccines Adjuvanted with Immunopotentiators in Chickens. Wu, Peipei,Lu, Jihu,Feng, Lei,Zhang, Xuehua,Mei, Mei,Hou, Jibo,Tang, Yinghua,Wu, Peipei,Lu, Jihu,Feng, Lei,Zhang, Xuehua,Mei, Mei,Hou, Jibo,Tang, Yinghua,Wu, Peipei,Lu, Jihu,Feng, Lei,Zhang, Xuehua,Mei, Mei,Hou, Jibo,Liu, Xiufan,Tang, Yinghua,Wu, Hongzhuan,Liu, Xiufan. 2017

[5]An inactivated gE-deleted pseudorabies vaccine provides complete clinical protection and reduces virus shedding against challenge by a Chinese pseudorabies variant. Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Gu, Yiqi,Liu, Chang,Hou, Jibo,Gu, Yiqi,Liu, Chang,Wang, Jichun,Guo, Rongli,Qiao, Yongfeng,Xu, Mengwei,Wang, Zhisheng,Liu, Yamei,Hou, Jibo. 2016

[6]CTA1: Purified and display onto gram-positive enhancer matrix (GEM) particles as mucosal adjuvant. Zhang, Yuanpeng,Yu, Xiaoming,Hou, Liting,Chen, Jin,Li, Pengcheng,Qiao, Xuwen,Zheng, Qisheng,Hou, Jibo,Zhang, Yuanpeng,Yu, Xiaoming,Hou, Liting,Chen, Jin,Li, Pengcheng,Qiao, Xuwen,Zheng, Qisheng,Hou, Jibo,Zhang, Yuanpeng,Yu, Xiaoming,Hou, Liting,Chen, Jin,Li, Pengcheng,Qiao, Xuwen,Zheng, Qisheng,Hou, Jibo. 2018

[7]Effects of Mycoplasma hyopneumoniae on porcine nasal cavity dendritic cells. Shen, Yumeng,Hu, Weiwei,Yang, Qian,Wei, Yanna,Feng, Zhixin.

[8]In vitro protective efficacy of Lithium chloride against Mycoplasma hyopneumoniae infection. Ishag, Hassan Z. A.,Wu, Yu-zi,Liu, Mao-jun,Xiong, Qi-yan,Feng, Zhi-xin,Yang, Ruo-song,Shao, Guo-qing,Ishag, Hassan Z. A..

[9]Transcription analysis on response of porcine alveolar macrophages to co-infection of the highly pathogenic porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Li, Bin,Du, Luping,Xu, Xiangwei,Sun, Bing,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang,Li, Bin,Yu, Zhengyu,Feng, Zhixin,Liu, Maojun,Wei, Yanna,Wang, Haiyan,Shao, Guoqing,He, Kongwang.

[10]The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells. Wang, Haiyan,Feng, Zhixin,Wu, Yuzi,Wei, Yanna,Gan, Yuan,Hua, Lizhong,Li, Bin,Wang, Xiaomin,Liu, Maojun,Xiong, Qiyan,Shao, Guoqing,Wang, Haiyan,Feng, Zhixin,Shao, Guoqing.

[11]CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae. Fang, Xiaomin,Zhao, Weimin,Xu, Jie,Tu, Feng,Wang, Xuemin,Li, Bixia,Fu, Yanfeng,Ren, Shouwen.

[12]Use of serological and mucosal immune responses to Mycoplasma hyopneumoniae antigens P97R1, P46 and P36 in the diagnosis of infection. Feng, Zhi-Xin,Bai, Yun,Yao, Jing-Ting,Gan, Yuan,Wang, Hai-Yan,Wei, Yan-Na,Liu, Mao-Jun,Xiong, Qi-Yan,Bai, Fang-Fang,Li, Bin,Wu, Xu-Su,Shao, Guo-Qing,Feng, Zhi-Xin,Bai, Yun,Liu, Mao-Jun,Shao, Guo-Qing,Pharr, G. Todd,Wan, Xiu-Feng,Xiao, Shao-Bo,Chi, Ling-Zhi.

[13]Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65. Liu, Maojun,Zhang, Yue,Wu, Yuzi,Wang, Haiyan,Li, Bin,Bai, Yun,Feng, Zhixin,Xiong, Qiyan,Bai, Fangfang,Shao, Guoqing,Liu, Maojun,Du, Gaimei,Browning, Glenn F.,Shao, Guoqing.

[14]Comparative analysis of mucosal immunity to Mycoplasma hyopneumoniae in Jiangquhai porcine lean strain and DLY piglets. Hua, L. Z.,Wu, Y. Z.,Bai, F. F.,William, K. K.,Feng, Z. X.,Liu, M. J.,Yao, J. T.,Zhang, X.,Shao, G. Q.. 2014

[15]A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene. Liu, M. J.,Du, G. M.,Bai, F. F.,Wu, Y. Z.,Xiong, Q. Y.,Feng, Z. X.,Li, B.,Shao, G. Q.,Du, G. M.,Liu, M. J.,Shao, G. Q.. 2015

[16]A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae. Ishag, H. Z. A.,Liu, M. J.,Yang, R. S.,Xiong, Q. Y.,Feng, Z. X.,Shao, G. Q.,Ishag, H. Z. A.. 2016

[17]Development and validation of an attenuated Mycoplasma hyopneumoniae aerosol vaccine. Feng, Zhi-Xin,Wei, Yan-Na,Li, Gui-Lan,Lu, Xiao-Ming,Wang, Zhan-Wei,Kong, Meng,Gan, Yuan,Bai, Fang-Fang,Liu, Mao-Jun,Xiong, Qi-Yan,Wu, Xu-Su,Shao, Guo-Qing,Wan, Xiu-Feng,Pharr, G. Todd. 2013

[18]Cholesterol exacerbates Mycoplasma hyopneumoniae-induced apoptosis via stimulating proliferation and adhesion to porcine alveolar macrophages. Liu, Maojun,Hu, Yun,Liu, Jie,Jia, Yimin,Zhao, Ruqian,Liu, Maojun,Liu, Beibei,Shao, Guoqing,Jia, Yimin,Zhao, Ruqian,Du, Gaimei,Minion, F. Chris,Shao, Guoqing. 2017

[19]Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain. Liu, Wei,Xiao, Shaobo,Li, Mao,Guo, Shaohua,Li, Sha,Luo, Rui,Chen, Huanchun,Fang, Liurong,Feng, Zhixin,Li, Bin,Shao, Guoqing,Zhou, Zhemin. 2013

[20]Apoptosis induced by lipid-associated membrane proteins from Mycoplasma hyopneumoniae in a porcine lung epithelial cell line with the involvement of caspase 3 and the MAPK pathway. Ni, B.,Bai, F. F.,Wei, Y.,Liu, M. J.,Feng, Z. X.,Xiong, Q. Y.,Hua, L. Z.,Shao, G. Q.. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台