您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus

文献类型： 外文期刊

作者： Shen, Wentao 1 ; Tuo, Decai 1 ; Yan, Pu 1 ; Yang, Yong 1 ; Li, Xiaoying 1 ; Zhou, Peng 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol & Anal, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Peoples R China; Chinese Acad Trop Agr Sci, Testing Ctr, Haikou 571101, Peoples R China

关键词： Papaya ringspot virus;PRSV detection;Reverse transcription loop-mediated isothermal amplification;RT-PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Papaya ringspot virus (PRSV) and Papaya leaf distortion mosaic virus (PLDMV), which causes disease symptoms similar to PRSV, threaten commercial production of both non-transgenic-papaya and PRSV-resistant transgenic papaya in China. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to detect PLDMV was developed previously. In this study, the development of another RT-LAMP assay to distinguish among transgenic, PRSV-infected and PLDMV-infected papaya by detection of PRSV is reported. A set of four RT-LAMP primers was designed based on the highly conserved region of the P3 gene of PRSV. The RT-LAMP method was specific and sensitive in detecting PRSV, with a detection limit of 1.15 x 10(-6) mu g of total RNA per reaction. Indeed, the reaction was 10 times more sensitive than one-step RT-PCR. Field application of the RT-LAMP assay demonstrated that samples positive for PRSV were detected only in non-transgenic papaya, whereas samples positive for PLDMV were detected only in commercialized PRSV-resistant transgenic papaya. This suggests that PRSV remains the major limiting factor for non-transgenic-papaya production, and the emergence of PLDMV threatens the commercial transgenic cultivar in China. However, this study, combined with the earlier development of an RT-LAMP assay for PLDMV, will provide a rapid, sensitive and cost-effective diagnostic power to distinguish virus infections in papaya

  • 相关文献

[1]Complete genome of Hainan papaya ringspot virus using small RNA deep sequencing. Zhang, Yuliang,Yu, Naitong,Huang, Qixing,Guo, Anping,Liu, Zhixin,Yin, Guohua,Wang, Xiangfeng,Xiong, Zhongguo,Wang, Xiangfeng,Xiong, Zhongguo.

[2]A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system. Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.. 2012

[3]Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system. Shen, W. T.,Wang, M. Q.,Yan, P.,Gao, L.,Zhou, P.. 2010

[4]Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China. Tuo, D.,Li, Ch,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Li, H.. 2013

[5]Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR. Huo, P.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P..

[6]Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria. Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Yang, G..

[7]Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.). Lv Lingling,Sun Guangming,Zhang Jianxia,Zeng Songjun,Duan Jun,Lv Lingling,Xie Jianghui,Liu Shenghui,Liu Yuge,Wei Changbin,Lv Lingling,Sun Guangming. 2011

[8]Detection of Papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification. Shen, Wentao,Tuo, Decai,Yan, Pu,Li, Xiaoying,Zhou, Peng,Shen, Wentao,Tuo, Decai,Yan, Pu,Li, Xiaoying,Zhou, Peng,Tuo, Decai. 2014

[9]甘蔗谷胱甘肽硫转移酶基因SoGST-1α的克隆与表达分析. 徐磊,姚艳丽,胡小文,邢淑莲,刘洋. 2015

[10]甜高粱WRKY转录因子基因的克隆与表达分析. 徐磊,胡小文,姚艳丽,邢淑莲,刘洋. 2017

[11]海南石斑鱼苗病毒性神经坏死病的初步诊断. 郭德权,费小雯,李亚军,肖洒,朱为菊,郑佩华,邓晓东. 2019

[12]康乃馨ACC氧化酶cDNA的克隆及其反义植物表达载体的构建. 张树珍,汤火龙,杨本鹏,刘飞虎. 2002

[13]海南方格星虫纤溶酶分离纯化及部分基因克隆. 马伏宁,谭琳,郭刚,臧小平,殷晓敏,王达新,马蔚红. 2018

[14]木薯总RNA提取初步研究. 张根良,王文泉. 2008

[15]番木瓜果实中芥子酶基因组织差异表达和酶活性研究. 农汉,孙雪飘,谭德冠,付莉莉,张家明. 2011

[16]木薯蔗糖合酶(SuSy)基因的表达分析及SuSy1和SuSy4编码序列的克隆. 方开星,陈新,王海燕,王淑娟,马平安,王文泉. 2014

[17]马铃薯花青素转录激活基因stwd40的克隆与表达分析. 罗遵喜,刘仕芸,张树珍,杨本鹏,蔡文伟. 2008

[18]巴西橡胶树6个蔗糖转运蛋白基因的克隆与序列分析. 阳江华,黄德宝,刘术金,唐朝荣. 2007

[19]马铃薯花青素转录激活基因(stmyc)的克隆及表达分析. 刘仕芸,张树珍,蔡文伟. 2009

[20]香蕉凝集素基因的克隆及在成熟果实中的特异性表达(英文). 金志强,张德有,徐碧玉. 2004

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台