您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge

文献类型： 外文期刊

作者： Sun, Shengming 1 ; Gu, Zhimin 1 ; Fu, Hongtuo 3 ; Zhu, Jian 3 ; Xuan, Fujun 4 ; Ge, Xianping 3 ;

作者机构： 1.Zhejiang Inst Freshwater Fisheries, Minist Agr, Key Lab Hlth Freshwater Aquaculture, Huzhou 313001, Peoples R China

2.Zhejiang Inst Freshwater Fisheries, Key Lab Fish Hlth & Nutr Zhejiang Prov, Huzhou 313001, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Genet Breeding & Aquaculture Biol Freshwa, Wuxi 214081, Peoples R China

4.Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 22

关键词： Ferritin;cDNA cloning;Gene expression;Iron;Macrobrachium nipponense

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ferritin, a major iron storage protein in most living organisms, plays a crucial role in iron metabolism. In this study, the ferritin subunit MnFer was identified in the oriental river prawn (Macrobrachium nipponense) and functionally characterized. The full-length cDNA of MnFer is 999 bp in size with a 122-bp 5'-untranslated region (UTR), a 364-bp 3'-UTR and a 513-bp open reading frame that encodes a protein possessing 171 amino acids and a deduced molecular weight of 19.40 kDa. Prawn ferritin transcripts are expressed in muscle, heart, hepatopancreas, gill, hemocytes, ovary and testis. Quantitative real-time PCR revealed that the abundance of ferritin transcript was highest in the hepatopancreas followed by muscle. Ferritin transcript expression in muscle increased six-fold 3 h after the injection of iron. In the gill, a fourfold increase in ferritin transcript expression was detected 3 h post-injection; the expression remained elevated for 48 h. Heart ferritin mRNA expression increased up to seven-fold at 24 h post-injection. No significant difference was found in the hepatopancreas. The iron binding capacity of recombinant ferritin protein was also demonstrated in this study. In hemocyte experiments, the transcriptional expression of MnFer showed the strongest response to Aeromonas hydrophila. As a whole, our study suggested that the ferritin from M. nipponense may play critical roles in cellular and organismic iron homeostasis along with in innate immune defense

  • 相关文献

[1]Molecular Characterization and Expression Analyses of the Complement Component C8 alpha, C8 beta and C9 Genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila Challenge. Zheng, Huan,Ji, Wei,Zhang, Gui-Rong,Zhang, Xiao-Ting,Wei, Kai-Jian,Yang, Rui-Bin,Gardner, Jonathan P. A.,Zheng, Huan,Ji, Wei,Zhang, Gui-Rong,Zhang, Xiao-Ting,Shi, Ze-Chao,Wei, Kai-Jian,Yang, Rui-Bin,Gardner, Jonathan P. A.,Shi, Ze-Chao,Gardner, Jonathan P. A.. 2016

[2]Molecular characterization and expression analysis of three TLR genes in yellow catfish (Pelteobagrus fulvidraco): Responses to stimulation of Aeromonas hydrophila and TLR ligands. Wang, Kai-Lun,Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Zhang, Xiao-Ting,Zheng, Huan,Fan, Qi-Xue,Wang, Kai-Lun,Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Shi, Ze-Chao,Zhang, Xiao-Ting,Zheng, Huan,Fan, Qi-Xue,Shi, Ze-Chao.

[3]Molecular characterization and mRNA expression of hypoxia inducible factor-1 and cognate inhibiting factor in Macrobrachium nipponense in response to hypoxia. Sun, Shengming,Fu, Hongtuo,Ge, Xianping,Zhu, Jian,Qiao, Hui,Jin, Shubo,Zhang, Wenyi,Xuan, Fujun.

[4]Molecular Cloning and Functional Characterization of a Hexokinase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun. 2017

[5]Transciptomic and histological analysis of hepatopancreas, muscle and gill tissues of oriental river prawn (Macrobrachium nipponense) in response to chronic hypoxia. Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun,Gu, Zhimin. 2015

[6]Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense. Zhang, W. Y.,Fu, H. T.,Zhang, W. Y.,Jiang, S. F.,Xiong, Y. W.,Fu, H. T.,Qiao, H.,Sun, S. M.,Gong, Y. S.,Jin, S. B.. 2015

[7]干露胁迫对脊尾白虾(Exopalaemon carinicauda)HSP70和ferritin基因表达的影响. 王琦,李健,李吉涛,潘鲁青,杨爱国. 2013

[8]Ferritin has an important immune function in the ark shell Scapharca broughtonii. Zheng, Libing,Liu, Zhihong,Wu, Biao,Zhou, Liqing,Tian, Jiteng,Sun, Xiujun,Yang, Aiguo,Zheng, Libing,Dong, Yinghui.

[9]Differential response of two ferritin subunit genes (VpFer1 and VpFer2) from Venerupis philippinarum following pathogen and heavy metals challenge. Jia, Xiaoping. 2013

[10]Isolation and Characterization of a Ferritin cDNA from the Mud Crab Scylla paramamosain. Zhang, Dan,Jiang, Keji,Zhang, Fengying,Ma, Chunyan,Shi, Yanhong,Qiao, Zhenguo,Ma, Lingbo,Zhang, Dan.

[11]Identification and involvement of ferritin in the response to pathogen challenge in the abalone, Haliotis diversicolor. He, Jian,Jiang, Jingzhe,Gu, Lu,Zhao, Manman,Wang, Ruixuan,Ye, Lingtong,Yao, Tuo,Wang, Jiangyong,Gu, Lu,Zhao, Manman.

[12]The effects of copper, iron and zinc on digestive enzyme activity in the hybrid tilapia Oreochromis niloticus (L.) x Oreochromis aureus (Steindachner). Li, J. -S.,Li, J. -L.,Wu, T. -T.. 2007

[13]Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus. Liu, Hong,Takano, Tomokazu,Abernathy, Jason,Wang, Shaolin,Sha, Zhenxia,Jiang, Yanliang,Terhune, Jeffery,Kucuktas, Huseyin,Peatman, Eric,Liu, Zhanjiang,Liu, Hong,Takano, Tomokazu,Abernathy, Jason,Wang, Shaolin,Sha, Zhenxia,Jiang, Yanliang,Terhune, Jeffery,Kucuktas, Huseyin,Peatman, Eric,Liu, Zhanjiang,Liu, Hong,Sha, Zhenxia. 2010

[14]Molecular cloning and expression analysis of Megalobrama amblycephala transferrin gene and effects of exposure to iron and infection with Aeromonas hydrophila. Teng, Tao,Xi, Bingwen,Xie, Jun,Xu, Pao,Teng, Tao,Xi, Bingwen,Xie, Jun,Chen, Kai,Xu, Pao,Pan, Liangkun.

[15]Cloning, characterization and expression of ferritin subunit from clam Meretrix meretrix in different larval stages. Wang, Xiaomei,Liu, Baozhong,Xiang, Jianhai,Wang, Xiaomei.

[16]Studies on resistance characteristic and cDNA sequence conservation of transferrin from crucian carp, Carassius auratus. Long, Hua,Yu, Qi-Xing.

[17]Characterization of -catenin 1 during the gonad development in the common carp (Cyprinus carpio). Wang, Lan-mei,Zhu, Wen-bin,Fu, Jian-jun,Dong, Zai-jie,Song, Fei-biao,Dong, Juan-juan,Dong, Zai-jie.

[18]Molecular cloning, characterization and mRNA expression of two antibacterial peptides: Crustin and anti-lipopolysaccharide factor in swimming crab Portunus trituberculatus. Yue, Feng,Pan, Luqing,Miao, Jingjing,Zhang, Lin,Li, Jian.

[19]The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri. Takano, Tomokazu,Sha, Zhenxia,Peatman, Eric,Terhune, Jeffery,Li, Hong,Kucuktas, Huseyin,Li, Ping,Liu, Zhanjiang,Sha, Zhenxia,Edholm, Eva-Stina,Wilson, Melanie.

[20]Polygenic expression of somatostatin in orange-spotted grouper (Epinephelus coioides): Molecular cloning and distribution of the mRNAs encoding three somatostatin precursors. Ying, Y,Li, WS,Lin, HR. 2005

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台