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Selection of an aptamer against rabies virus: A new class of molecules with antiviral activity

文献类型： 外文期刊

作者： Liang, Hong-Ru 1 ; Hu, Gui-Qiu 2 ; Xue, Xiang-Hong 2 ; Li, Lu 2 ; Zheng, Xue-Xing 2 ; Gao, Yu-Wei 2 ; Yang, Song-Tao 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Fishery Drug Dev, Minist Agr, Guangzhou, Guangdong, Peoples R China

2.Acad Mil Med Sci, Inst Mil Vet, Changchun 130062, Peoples R China

关键词： Aptamer;Rabies virus;Therapeutic agents

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： Rabies is a fatal central nervous system (CNS) disease caused by the neurotropic rabies virus (RABV). The therapeutic management of RABV infections is still problematic, and novel antiviral strategies are urgently required. We established the RVG-BHK-21 cell line, which expresses RABV glycoprotein on the cell surface, to select aptamers. Through 28 iterative rounds of selection, single-stranded DNA (ssDNA) aptamers were generated by exponential enrichment (SELEX). A virus titer assay and a real-time quantitative reverse transcription PCR (qRT-PCR) assay revealed that four aptamers could inhibit the replication of RABV in cultured baby hamster kidney (BHK)-21 cells. However, the aptamers did not inhibit the replication of other virus, e.g., canine distemper virus (CDV) and canine parvovirus (CPV). In addition, the GE54 aptamer was found to effectively protect mice against lethal RABV challenge. After inoculation with aptamers for 24 h or 48 h, followed by inoculation with CVS-11, approximately 25-33% of the mice survived. In summary, we selected aptamers that could significantly protect from a lethal dose of RABV in vitro and in vivo.

  • 相关文献

[1]Aptamers targeting rabies virus-infected cells inhibit street rabies virus in vivo. Liang, Hong-Ru,Liang, Hong-Ru,Hu, Gu-Qiu,Li, Ling,Gao, Yu-Wei,Yang, Song-Tao,Xia, Xian-Zhu. 2014

[2]A novel electrochemical PCB77-binding DNA aptamer biosensor for selective detection of PCB77. Wu, Lidong,Fu, Xiaochen,Liu, Huan,Li, Jincheng,Wang, Qiong,Fan, Hao,Qi, Peipei.

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