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Identification and Functional Analysis of foxo Genes in Chinese Tongue Sole (Cynoglossus semilaevis)

文献类型： 外文期刊

作者： Zhang, Tingting 1 ; Zhang, Mengqian 1 ; Sun, Yuxuan 1 ; Li, Lu 1 ; Cheng, Peng 1 ; Li, Xihong 1 ; Wang, Na 1 ; Chen, Songlin 1 ; Xu, Wenteng 1 ;

作者机构： 1.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Lab Marine Fisheries Sci & Food Prod Proc, Pilot Natl Lab Marine Sci & Technol, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Cynoglossus semilaevis; foxo; promoter activity; siRNA knockdown

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 8 期

页码：

收录情况： SCI

摘要： The Chinese tongue sole (Cynoglossus semilaevis) is a traditional, precious fish in China. Due to the large growth difference between males and females, the investigation of their sex determination and differentiation mechanisms receives a great deal of attention. Forkhead Box O (FoxO) plays versatile roles in the regulation of sex differentiation and reproduction. Our recent transcriptomic analysis has shown that foxo genes may participate in the male differentiation and spermatogenesis of Chinese tongue sole. In this study, six Csfoxo members (Csfoxo1a, Csfoxo3a, Csfoxo3b, Csfoxo4, Csfoxo6-like, and Csfoxo1a-like) were identified. Phylogenetic analysis indicated that these six members were clustered into four groups corresponding to their denomination. The expression patterns of the gonads at different developmental stages were further analyzed. All members showed high levels of expression in the early stages (before 6 months post-hatching), and this expression was male-biased. In addition, promoter analysis found that the addition of C/EBP alpha and c-Jun transcription factors enhanced the transcriptional activities of Csfoxo1a, Csfoxo3a, Csfoxo3b, and Csfoxo4. The siRNA-mediated knockdown of the Csfoxo1a, Csfoxo3a, and Csfoxo3b genes in the testicular cell line of Chinese tongue sole affected the expression of genes related to sex differentiation and spermatogenesis. These results have broadened the understanding of foxo's function and provide valuable data for studying the male differentiation of tongue sole.

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