Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody
文献类型： 外文期刊
作者： Liua, Zhen 1 ; Zhi, Aimin 1 ; Zhao, Lina 1 ; Yang, Yanyan 1 ; Hu, Xiaofei 1 ; Deng, Ruiguang 1 ; Li, Qingwang 4 ; Zhang, 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou, Henan, Peoples R China
2.Henan Univ Technol, Coll Biotechnol, Zhengzhou 450000, Henan, Peoples R China
3.Northwest Agr & Forest Univ, Coll Anim Sci & Technol, Yangling, Shanxi, Peoples R China
4.No
期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）
ISSN：
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收录情况： SCI
摘要： A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed using a new monoclonal antibody for detecting the food colourant Sudan I. The half-maximum inhibition concentrations (IC50) and the limit of detection (calculated as IC20) of ELISA for Sudan I were 2 and 0.01 ng mL(-1), respectively. The study showed little cross-activity with Sudan I structural analogues (below 0.01%). The average recoveries in intra- and interassays for Sudan I from fortified fresh tomato and chilli samples by ELISA were in ranges of 82-94% and 79-91%, respectively. The coefficients of variation of intra- and interassays were 6-8% and 6-10%, respectively. The IC50 was approximately 2.0 ng mL(-1) after the Sudan I-Kit had been kept for 180 days
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