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Biofilm formation in Haemophilus parasuis: relationship with antibiotic resistance, serotype and genetic typing

文献类型： 外文期刊

作者： Zhang, Jianmin 1 ; Xu, Chenggang 1 ; Shen, Haiyan 2 ; Li, Jingyi 1 ; Guo, Lili 1 ; Cao, Guojie 3 ; Feng, Saixiang 1 ; Lia 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Key Lab Zoonoses Prevent & Control Guangdong, Key Lab Anim Dis Control & Prevent,Minist Agr, Guangzhou, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Guangdong, Peoples R China

3.Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： Biofilms are surface-associated microbial communities, which are encased in self-synthesized extracellular environment. Biofilm formation may trigger drug resistance and inflammation, resulting in persistent infections. Haemophilus parasuis is the etiological agent of a systemic disease, Glasser's disease, characterized by fibrinous polyserositis, arthritis and meningitis in pigs. The purpose of this study was to examine the correlation between biofilm and antibiotic resistance among the clinical isolates of H. parasuis. In the present study, we tested biofilm-forming ability of 110 H. parasuis isolates from various farms using polystyrene microtiter plate assays. Seventy-three isolates of H. parasuis (66.4%) showed biofilm formation and most of them performed weak biofilm-forming ability (38/73). All isolates were tested for antimicrobial susceptibility to 18 antimicrobial agents by the broth microdilution method. H. parasuis isolates showed very high resistance (>90%) to sulfanilamide, nalidixic acid, and trimethoprim. Resistance to eight antibiotics such as penicillin (41.1% vs 8.1%), ampicillin (31.5% vs 8.1%), amoxicillin (28.8% vs 5.4%), gentamicin (46.6% vs 24.3%), cefazolin (19.2% vs 2.7%), doxycycline (19.2% vs 8.1%), cefotaxime (11% vs 2.7%), and cefaclor (13.7% vs 5.4%) was comparatively higher among biofilm producers than non-biofilm producers. Pulsed-field gel electrophoresis (PFGE) analyses could distinguish various isolates. Our data indicated that H. parasuis field isolates were able to form biofilms in vitro. In addition, biofilm positive strains had positive correlation with resistance to beta-lactams antibiotics. Thus, biofilm formation may play important roles during H. parasuis infections. (C) 2014 Published by Elsevier Ltd.

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