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Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

文献类型： 外文期刊

作者： Wu, Li-ming 1 ; Guo, Rui 2 ; Hui, Lin 1 ; Ye, Yong-gang 3 ; Xiang, Jing-mei 1 ; Wan, Chun-yun 1 ; Zou, Miao 1 ; Ma, Rui 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

2.Hubei Acad Agr Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

3.Sichan Anim Sci Acad, Biotechnol Inst Anim & Vet Sci, Chengdu 610066, Peoples R China

关键词： Bc1-2;chronic enteritis;oxidative damage;stanniocalcin-1

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

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收录情况： SCI

摘要： Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (11202) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 mu M H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, overexpression of STC-1 up-regulated Bc1-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.

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[1]Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells. Xiang, Jinmei,Wan, Chunyun,Wu, Liming,Yang, Shijin,Guo, Dingzong,Xiang, Jinmei,Guo, Rui,Wan, Chunyun. 2016

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