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Separation and purification of amygdalin from thinned bayberry kernels by macroporous adsorption resins

文献类型： 外文期刊

作者： Wang, Tao 1 ; Lu, Shengmin 1 ; Xia, Qile 1 ; Fang, Zhongxiang 3 ; Johnson, Stuart 3 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, Key Lab Fruits & Vegetables Postharvest & Proc Zh, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Normal Univ, Jinhua 321000, Peoples R China

3.Curtin Univ, Int Inst Agrifood Secur IIAFS, Fac Hlth Sci, Food Sci & Technol Program,Sch Publ Hlth, Perth, WA 6845, Australia

关键词： Bayberry;Amygdalin;Thinned bayberry kernels (TBKs);Macroporous adsorption resins (MARS);Purification

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： To utilize the low-value thinned bayberry (Myrica rubra Sieb. et Zucc) kernels (TBKs) waste, an efficient method using macroporous adsorption resins (MARs) for separation and purification of amygdalin from TBKs crude extracts was developed. An aqueous crude sample was prepared from a methanol TBK extract, followed by resin separation. A series of MARs were initially screened for adsorption/desorption of amygdalin in the extract, and D101 was selected for characterization and method development. The static adsorption data of amygdalin on 0101 was best fitted to the pseudo-second-order kinetics model. The solute affinity toward D101 at 30 degrees C was described and the equilibrium experimental data were well-fitted to Langmuir and Freundlich isotherms. Through one cycle of dynamic adsorption/desorption, the purity of amygdalin in the extract, determined by HPLC, increased about 17-fold from 4.8% to 82.0%, with 77.9% recovery. The results suggested that D101 resin effectively separate amygdalin from TBKs. (C) 2014 Elsevier B.V. All rights reserved.

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