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Characterization and cross-species amplification of 19 polymorphic microsatellite loci of Hemibagrus macropterus (Bleeker) in the Yangtze River

文献类型： 外文期刊

作者： Ba, Jiawen 1 ; Li, Yun 1 ; Jia, Xiangyang 2 ; Wang, Dengqiang 2 ; Duan, Xinbin 2 ; Chen, Daqing 2 ;

作者机构： 1.Southwest Univ, Coll Anim Sci & Technol, Chongqing 400716, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

关键词： Hemibagrus macropterus;Microsatellite;Genetic marker

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

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收录情况： SCI

摘要： Hemibagrus macropterus (Bleeker) is endemic to the upper Yangtze River basin and the Zhujiang River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified nineteen microsatellite loci in a wild population. The number of alleles per locus ranged from 5 to 17. The observed and expected heterozygosity ranged from 0.5132 to 1.000 and from 0.4903 to 0.9008, respectively. Cross-amplification was tested in related species Leiocassis tenuifurcatus and Pelteobagrus fulvidraco.

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