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Rapid Visual Detection of Aflatoxin B1 by Label-Free Aptasensor Using Unmodified Gold Nanoparticles

文献类型： 外文期刊

作者： Luan, Yunxia 1 ; Chen, Zhengbo 2 ; Xie, Gang 3 ; Chen, Jiayi 1 ; Lu, Anxiang 1 ; Li, Cheng 1 ; Fu, Hailong 1 ; Ma, Zhihon 1 ;

作者机构： 1.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China

3.Acad State Adm Grain, Beijing 100037, Peoples R China

关键词： Aflatoxin B1 (AFB1);Aptamer;Label-Free;Gold Nanoparticles

期刊名称：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY （ 影响因子：1.134； 五年影响因子：0.999 ）

ISSN：

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收录情况： SCI

摘要： The selective detection of ultra trace amounts of aflatoxin B1 (AFB1) is extremely important for food safety, since it is the most toxic mycotoxin class that is allowed to be present in edible food and agricultural products with strictly Maximum Residue Limit (MRL). Sensitive detection of AFB1 residues requires time-consuming techniques and expensive instruments. An aptasensor for AFB1 detection, using unmodified gold nanoparticles (AuNPs) indicator, was developed in the present study, based on the salt-induced AuNPs aggregation phenomenon. Its linear dynamic range and detection sensitivity were found to be 0.025 ng/mL-100 ng/mL and 0.025 ng/mL of AFB1 respectively, which were lower than the maximum residue limit (MRL) in edible food, as set by China and the European Commission. Our study provides a simple, fast, and visible method for AFB1 analysis, with high sensitivity, which can be applied in future on-site detection for food and agricultural products.

  • 相关文献

[1]Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Xie, Gang. 2015

[2]Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles. Luan, Yunxia,Chen, Jiayi,Li, Cheng,Ping, Hua,Ma, Zhihong,Lu, Anxiang,Xie, Gang.

[3]A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li,Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li. 2016

[4]An oligosorbent-based aptamer affinity column for selective extraction of aflatoxin B-2 prior to HPLC with fluorometric detection. Liu, Hongmei,Luan, Yunxia,Lu, Anxiang,Li, Bingru,Wang, JiHua,Yang, Meihua,Yang, Meihua. 2018

[5]Superior fluorescent probe for detection of potassium ion. Chen, Zhengbo,Tan, Lulu,Hu, Liangyu,Luan, Yunxia.

[6]Chemiluminescence assay for detection of 2-hydroxyfluorene using the G-quadruplex DNAzyme-H2O2-luminol system. Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Li, An,Jin, Xinxin,Pan, Ligang,Liang, Gang,Liu, Xinhui. 2018

[7]Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy. Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Man, Yan,Jin, Xinxin,Pan, Ligang,Liang, Gang,Liu, Xinhui.

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