Rapid Visual Detection of Aflatoxin B1 by Label-Free Aptasensor Using Unmodified Gold Nanoparticles
文献类型： 外文期刊
作者： Luan, Yunxia 1 ; Chen, Zhengbo 2 ; Xie, Gang 3 ; Chen, Jiayi 1 ; Lu, Anxiang 1 ; Li, Cheng 1 ; Fu, Hailong 1 ; Ma, Zhihon 1 ;
作者机构： 1.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
2.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
3.Acad State Adm Grain, Beijing 100037, Peoples R China
关键词： Aflatoxin B1 (AFB1);Aptamer;Label-Free;Gold Nanoparticles
期刊名称：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY （ 影响因子：1.134； 五年影响因子：0.999 ）
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收录情况： SCI
摘要： The selective detection of ultra trace amounts of aflatoxin B1 (AFB1) is extremely important for food safety, since it is the most toxic mycotoxin class that is allowed to be present in edible food and agricultural products with strictly Maximum Residue Limit (MRL). Sensitive detection of AFB1 residues requires time-consuming techniques and expensive instruments. An aptasensor for AFB1 detection, using unmodified gold nanoparticles (AuNPs) indicator, was developed in the present study, based on the salt-induced AuNPs aggregation phenomenon. Its linear dynamic range and detection sensitivity were found to be 0.025 ng/mL-100 ng/mL and 0.025 ng/mL of AFB1 respectively, which were lower than the maximum residue limit (MRL) in edible food, as set by China and the European Commission. Our study provides a simple, fast, and visible method for AFB1 analysis, with high sensitivity, which can be applied in future on-site detection for food and agricultural products.
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