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Complete nucleotide sequence of Klebsiella phage P13 and prediction of an EPS depolymerase gene

文献类型： 外文期刊

作者： Shang, Anqi 1 ; Liu, Yang 1 ; Wang, Jianlei 1 ; Mo, Zhaolan 2 ; Li, Guiyang 2 ; Mou, Haijin 1 ;

作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Klebsiella phage P13;Complete genome sequencing;Predicted genes;Genomic comparison;EPS depolymerase

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

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收录情况： SCI

摘要： The complete genome of Klebsiella phage P13 was sequenced and analyzed. Bacteriophage P13 has a double-stranded linear DNA with a length of 45,976 bp and a G+C content of 51.7 %, which is slightly lower than that of Klebsiella pneumoniae KCTC 2242. The codon biases of phage P13 are very similar to those of SP6-like phages and K. pneumoniae KCTC 2242. Bioinformatics analysis shows that the phage P13 genome has 282 open reading frames (ORFs) that are greater than 100 bp in length, and 50 of these ORFs were identified as predicted genes with an average length of 833 bp. Among these genes, 41 show homology to known proteins in the GenBank database. The functions of the 24 putative proteins were investigated, and 13 of these were found to be highly conserved. According to the homology analysis of the 50 predicted genes and the whole genome, phage P13 is homologous to SP6-like phages. Furthermore, the morphological characteristics of phage P13 suggest that it belongs to the SP6-like viral genus of the Podoviridae subfamily Autographivirinae. Two hypothetical genes encoding an extracellular polysaccharide depolymerase were predicted using PSI-BLAST. This analysis serves as groundwork for further research and application of the enzyme.

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