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Development and characterization of neutralizing monoclonal antibodies against canine distemper virus hemagglutinin protein

文献类型： 外文期刊

作者： Bi, Zhenwei 1 ; Xia, Xingxia 1 ; Wang, Yongshan 1 ; Mei, Yongjie 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr,Natl Ctr Engn Res Vet Bioprod, Nanjing 210014, Peoples R China

2.Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

关键词： antigenic variation;canine distemper virus;monoclonal antibody;neutralizing activity

期刊名称：MICROBIOLOGY AND IMMUNOLOGY （ 影响因子：1.955； 五年影响因子：1.917 ）

ISSN：

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收录情况： SCI

摘要： Canine distemper virus (CDV) causes a serious multisystemic disease in dogs and other carnivora. Hemagglutinin (H) protein-specific antibodies are mainly responsible for protective immunity against CDV infection. In the present study, six neutralizing MAbs to the H protein of CDV were newly obtained and characterized by immunizing BALB/c mice with a recent Chinese field isolate. Competitive binding inhibition assay revealed that they recognized four distinct antigenic regions of the H protein. Immunofluorescence assay and western blotting showed that all MAbs recognize the conformational rather than the linear epitopes of the H protein. Furthermore, in immunofluorescence and virus neutralization assays, two of the MAbs were found to react only with the recent Chinese field isolate and not with older CDV strains, including vaccine strain Onderstepoort, indicating there are neutralization-related antigenic variations between the recent Chinese field isolate and the older CDV strains examined in this study. The newly established MAbs are useful for differentiating the expanding CDV strains and could be used in immunotherapy and immunodiagnosis against infection with CDV.

  • 相关文献

[1]Development of CDV-specific monoclonal antibodies for differentiation of variable epitopes of nucleocapsid protein. Bi, Zhenwei,Wang, Yongshan,Pan, Qunxing,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Pan, Qunxing,Xia, Xingxia,Xu, Libo.

[2]Phylogenetic analysis of canine distemper virus in domestic dogs in Nanjing, China. Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia,Bi, Zhenwei,Wang, Yongshan,Wang, Xiaoli,Xia, Xingxia.

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