您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

RNA-Seq analysis of differentially expressed genes in rice under varied nitrogen supplies

文献类型： 外文期刊

作者： Yang, Shun-ying 1 ; Hao, Dong-li 1 ; Song, Zhi-zhong 3 ; Yang, Guang-zhe 1 ; Wang, Li 1 ; Su, Yan-hua 1 ;

作者机构： 1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210008, Peoples R China

关键词： Rice;Ammonium;RNA-Seq;Differentially expressed genes

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ammonium is the main inorganic nitrogen source in paddy soil. Rice (Oryza sativa), an ammonium-preferring and -tolerant grain crop, is a valuable resource for researching ammonium-uptake mechanism and understanding the molecular networks that the plant copes with ammonium variation. To generate a broad survey of early responses affected by varied ammonium supplies in rice. RNA samples were prepared from the roots and shoots of rice plants subjected to nitrogen-free (0 mM ammonium), 1 mM ammonium and high ammonium (10 mM ammonium) for a short period of 4 h (1 mM ammonium treatment as the control), respectively, and the transcripts were sequenced using the Illumina/HiSeq (TM) 2000 RNA sequencing (RNA-Seq) platform. By comparative analysis, 394 differentially expressed genes (DEGs) were identified in roots, among which, 143 and 251 DEGs were up- and down-regulated under nitrogen-free condition, respectively. In shoots, 468 (119 up-regulated/ 349 down-regulated) DEGs were found under such condition. However, with high ammonium treatment, only 63 genes (6 up-regulated/57 down-regulated) in roots and 115 genes in shoots (93 up-regulated/22 down-regulated) were differentially expressed. According to KEGG analysis, when exposed to nitrogen-free condition, DEGs participating in the carbohydrate and amino acid metabolisms were down-regulated (with 1 exception) in roots as well as in shoots, implying reduced carbohydrate and nitrogen metabolisms. Under high ammonium supply, all DEGs associated with carbohydrate and amino acid metabolisms were down-regulated in roots and to the contrary, up-regulated in shoots. Aldehyde dehydrogenase (ALDH, NAD(+)) [EC: 1.2.13] seemed to have played an important role in rice shoots under high ammonium condition, analysis results implicated a coordinative regulation of carbohydrate with amino acid metabolisms under nitrogen deficiency as well as the high ammonium conditions during a short period of several hours in rice. Moreover, transcripts with abundance variation might be precious gene resources in responding to different ammonium supplies in rice. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Aluminium tolerance in rice is antagonistic with nitrate preference and synergistic with ammonium preference. Zhao, Xue Qiang,Shen, Ren Fang,Guo, Shi Wei,Shinmachi, Fumie,Sunairi, Michio,Noguchi, Akira,Hasegawa, Isao.

[2]痛风雏鹅肾组织损伤的RNA-seq分析. 邵春荣,黄远丕,黄运茂,应诗家,奚雨萌,施振旦. 2020

[3]基于RNA-seq的两种柳树转录组微卫星特征比较分析. 郑纪伟,周洁,王保松,何旭东. 2019

[4]玉米Glyco-hydro-16糖苷酶家族全基因组的鉴定及其遗传分化. 林峰,葛敏,周玲,赵涵. 2016

[5]热应激下公兔睾丸组织形态和精液转录组分析. 蔡佳炜,张琛,靳荣帅,鲍志远,张希宇,王璠,翟频,赵博昊,陈阳,汤先伟,吴信生. 2023

[6]利用RNA-seq技术分析淹水胁迫下转BnERF拟南芥差异表达基因. 吕艳艳,付三雄,陈松,张唯,戚存扣. 2015

[7]基于海甘蓝RNA-Seq序列开发EST-SSR分子标记. 戚维聪,程计华,黄邦全,李坦,林峰. 2014

[8]Root-applied brassinolide can alleviate the NaCl injuries on cotton. Shu, Hongmei,Ni, Wanchao,Guo, Shugiao,Gong, Yuanyong,Shen, Xinlian,Zhang, Xianggui,Xu, Peng,Guo, Qi.

[9]RNA-seq analysis reveals a key role of brassinolide-regulated pathways in NaCl-stressed cotton. Shu, H. M.,Guo, S. Q.,Gong, Y. Y.,Jiang, L.,Zhu, J. W.,Ni, W. C.. 2017

[10]Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.). Sun, Xiaochuan,Xu, Liang,Wang, Yan,Luo, Xiaobo,Kinuthia, Karanja Benard,Nie, Shanshan,Feng, Haiyang,Li, Chao,Liu, Liwang,Sun, Xiaochuan,Xu, Liang,Wang, Yan,Nie, Shanshan,Liu, Liwang,Zhu, Xianwen.

[11]Transcriptome and digital gene expression analysis of herbaceous peony (Paeonia lactiflora Pall.) to screen thermo-tolerant related differently expressed genes. Hao, Zhaojun,Tao, Jun,Hao, Zhaojun,Wei, Mengran,Gong, Saijie,Zhao, Daqiu,Tao, Jun,Zhao, Daqiu,Tao, Jun.

[12]De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress. Xu, Hai,Song, Bo,Chen, Jinfeng,Xu, Hai,Chen, Longzheng,Song, Bo,Fan, Xiaoxue,Yuan, Xihan.

[13]Comprehensive analysis of differentially expressed genes under salt stress in pear (Pyrus betulaefolia) using RNA-Seq. Li, Hui,Lin, Jing,Yang, Qing-Song,Li, Xiao-Gang,Chang, You-Hong.

[14]Comparative transcriptome analysis of fruiting body and sporulating mycelia of Villosiclava virens reveals genes with putative functions in sexual reproduction. Yu, Jun-Jie,Yu, Mi-Na,Nie, Ya-Feng,Yin, Xiao-Le,Zhao, Jie,Wang, Ya-Hui,Ding, Hui,Qi, Zhong-Qiang,Du, Yan,Liu, Yong-Feng,Sun, Wen-Xian,Sun, Wen-Xian,Huang, Li.

[15]De novo transcriptome sequencing of Acer palmatum and comprehensive analysis of differentially expressed genes under salt stress in two contrasting genotypes. Rong, Liping,Li, Qianzhong,Li, Shushun,Tang, Ling,Wen, Jing.

[16]Transcriptome sequencing analysis of porcine granulosa cells treated with an anti-inhibin antibody. Lei, Mingming,Cai, Liuping,Li, Hui,Chen, Zhen,Shi, Zhendan. 2017

[17]Systemic analysis of gene expression profiles in porcine granulosa cells during aging. Li Hui,Guo Shuangshuang,Yu Jianning,Shi Zhendan. 2017

[18]Transcriptome Analysis of Brassica rapa Near-lsogenic Lines Carrying Clubroot-Resistant and -Susceptible Alleles in Response to Plasmodiophora brassicae during Early Infection. Chen, Jingjing,Pang, Wenxing,Piao, Zhongyun,Chen, Bing,Zhang, Chunyu,Zhang, Chunyu. 2016

[19]Transcriptome sequencing and analysis of major genes involved in calcium signaling pathways in pear plants (Pyrus calleryana Decne.). Lin, Jing. 2015

[20]UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot. Wu, Xinxin,Ni, Xiaopeng,Zhou, Yong,Gao, Zhihong,Wu, Xinxin,Gong, Qinghua. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台