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Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test

文献类型： 外文期刊

作者： Wang, Yao 1 ; Deng, Ruiguang 2 ; Zhang, Gaiping 2 ; Li, Qingmei 2 ; Yang, Jifei 2 ; Sun, Yaning 2 ; Li, Zhixi 1 ; Hu, Xia 1 ;

作者机构： 1.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China

2.Henan Acad Agr Sci, Henan Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China

关键词： glycinin;immunochromatographic strip;colloidal gold;immunoassay

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A rapid immunochromatographic lateral flow test strip in a sandwich format was developed with the colloidal gold-labeled mouse antiglycinin monoclonal antibody (mAb) and rabbit antiglycinin polyclonal antibody (pAb) to specifically identify glycinin, a soybean allergen. The test strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad, and a test membrane containing a control line and a test line. This test strip has high sensitivity, and results can be obtained within 10 min without sophisticated procedures. The limit of detection (LOD) of the test strip was calculated to be 0.69 mg/kg using an optical density scanner that measures relative optical density. The assay showed high specificity for glycinin, with no cross-reactions with other soybean proteins or other food allergens. The recoveries of the lateral flow test strip in detecting glycinin in powdered milk samples ranged between 80.5 and 89.9% with relative standard deviations of less than 5.29% (intra-assay) and 6.72% (interassay). Therefore, the test strip is useful as a quantitative, semiquantitative, or qualitative detection method for glycinin in powdered milk. In addition, the test strip can be used to detect glycinin in other processed foods and may be a valuable tool in identifying effective approaches for reducing the impact of glycinin.

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