您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Analysis of differential gene expression under low-temperature stress in Nile tilapia (Oreochromis niloticus) using digital gene expression

文献类型： 外文期刊

作者： Yang, Changgeng 1 ; Jiang, Ming 1 ; Wen, Hua 1 ; Tian, Juan 1 ; Liu, Wei 1 ; Wu, Fan 1 ; Gou, Gengwu 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodiveis Conservat & Utilizat, Wuhan 430223, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodiveis

关键词： Digital gene expression;Tilapia;Low-temperature stress

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Tilapia (Oreochromis niloticus) do not survive well at low temperatures. Therefore, improvement of the low-temperature resistance has become an important issue for aquaculture development of tilapia. The objective of this study was to construct a digital gene expression tag profile to identify genes potentially related to low temperature in tilapia. In this study, tilapia was treated at 30 degrees C to lethal temperature 10 degrees C in decrement of 1 degrees C D-1. Digital gene expression analysis was performed using the Illumina technique to investigate differentially expressed genes in tilapia cultured at different temperatures (30 degrees C, 26 degrees C, 20 degrees C, 16 degrees C, and 10 degrees C). A total of 206,861, 188,082, 185,827, 188,067, and 214,171 distinct tags were obtained by sequencing these five libraries, respectively. Compared with the 30 degrees C library, there were 304, 407, 709, and 772 upregulated genes and 342, 793, 771, and 1466 downregulated genes in 26 degrees C, 20 degrees C, 16 degrees C, and 10 degrees C libraries, respectively. Trend analysis of these differentially expressed genes identified six statistically significant trends. Functional annotation analysis of the differentially expressed genes identified various functions associated with the response to low-temperature stress. When tilapia are subjected to low-temperature stress, expression changes were observed in genes associated with nucleic acid synthesis and metabolism, amino acid metabolism and protein synthesis, lipid and carbohydrate content and types, material transport, apoptosis, and immunity. The differentially expressed genes obtained in this study may provide useful insights to help further understand the effects of low temperature on tilapia. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Studies on fish heterosis with DNA fingerprinting. Wang, J,Xia, D.

[2]Growth arrest specific gene 2 in tilapia (Oreochromis niloticus): molecular characterization and functional analysis under low-temperature stress. Yang, ChangGeng,Wu, Fan,Lu, Xing,Jiang, Ming,Liu, Wei,Yu, Lijuan,Tian, Juan,Wen, Hua. 2017

[3]A global transcriptional analysis of Megalobrama amblycephala revealing the molecular determinants of diet-induced hepatic steatosis. Zhang, Dingdong,Lu, Kangle,Jiang, Guangzhen,Liu, Wenbin,Tian, Hongyan,Li, Xiangfei,Zhang, Dingdong,Dong, Zaijie.

[4]Transcriptome-wide analysis reveals candidate genes responsible for the asymmetric pigment pattern in scallop Patinopecten yessoensis. Sun, X. J.,Zhou, L. Q.,Liu, Z. H.,Wu, B.,Yang, A. G.. 2016

[5]The effects of copper, iron and zinc on digestive enzyme activity in the hybrid tilapia Oreochromis niloticus (L.) x Oreochromis aureus (Steindachner). Li, J. -S.,Li, J. -L.,Wu, T. -T.. 2007

[6]Preliminary fractionation and derivatives changes of myoglobin from tilapia (O. niloticus female xO. aureus male) dark muscle with ammonium sulfate. Cen, Jianwei,Hao, Shuxian,Li, Laihao,Yang, Xianqing,Hui, Huang,Hong, Shi,Wei, Ya,He, Junyan. 2012

[7]Microbiological, chemical and sensory assessment of (I) whole ungutted, (II) whole gutted and (III) filleted tilapia (Oreochromis niloticus) during refrigerated storage. Cao Rong,Liu Qi,Yin Bang-zhong,Cao Rong,Xue Chang-hu. 2009

[8]An outbreak of granulomatous inflammation associated with Francisella noatunensis subsp orientalis in farmed tilapia (Oreochromis niloticus x O-aureus) in China. Lin Qiang,Li Ningqiu,Fu Xiaozhe,Hu Qiandong,Chang Ouqin,Liu Lihui,Zhang Defeng,Wang Guangjun,Wu Shuqin,Lin Qiang,Li Ningqiu,Fu Xiaozhe,Liu Lihui,Zhang Defeng,Wu Shuqin,San Guibao. 2016

[9]Single dose pharmacokinetic study of flofenicol in tilapia (Oreochromis niloticus x O. aureus) held in freshwater at 22 degrees C. Feng, Jing-Bin,Feng, Jing-Bin,Feng, Jing-Bin,Jia, Xiao-Ping. 2009

[10]Ferulic acid: A natural compound as an efficient feed additive for GIFT (Oreochromis niloticus). Yu, L-J,Wu, F.,Jiang, M.,Yang, C-G,Liu, W.,Tian, J.,Lu, X.,Wen, H.,Yu, L-J. 2018

[11]The effects of modified atmosphere packaging and enzyme inhibitors on protein oxidation of tilapia muscle during iced storage. He, Yanfu,Li, Laihao,He, Yanfu,Huang, Hui,Li, Laihao,Yang, Xianqing,Hao, Shuxian,Chen, Shengjun,Deng, Jianchao. 2018

[12]Immunogenicity of the LrrG protein encapsulated in PLGA microparticles in Nile tilapia (Oreochromis niloticus) vaccinated against Streptococcus agalactiae. Ke, Xiaoli,Chen, Xue,Liu, Zhigang,Lu, Maixin,Gao, Fengying,Cao, Jianmeng,Chen, Xue. 2017

[13]Optimisation of extraction conditions and characteristics of skin gelatin from Nile tilapia (Oreochromis niloticus). Yan, Xiaoyan,Cao, Wenhong,Hong, Pengzhi,Zhang, Chaohua,Li, Laihao. 2010

[14]Dietary phosphatidylcholine impacts on growth performance and lipid metabolism in adult Genetically Improved Farmed Tilapia (GIFT) strain of Nile tilapia Oreochromis niloticus. Tian, Juan,Wen, Hua,Lu, Xing,Liu, Wei,Wu, Fan,Yang, Chang-Geng,Jiang, Ming,Yu, Li-Juan. 2018

[15]Recovery and pathogenicity analysis of Aerococcus viridans isolated from tilapia (Orecohromis niloticus) cultured in southwest of China. Ke, Xiaoli,Lu, Maixin,Ye, Xing,Gao, Fengying,Zhu, Huaping,Huang, Zhanghan. 2012

[16]Comparative proteome analysis of two Streptococcus agalactiae strains from cultured tilapia with different virulence. Li, Wei,Su, You-Lu,Mai, Yong-Zhan,Li, Yan-Wei,Li, An-Xing,Su, You-Lu,Mo, Ze-Quan.

[17]Dietary phosphorus requirement of GIFT strain of Nile tilapia Oreochromis niloticus reared in freshwater. Yao, Y. F.,Jiang, M.,Wen, H.,Wu, F.,Liu, W.,Tian, J.,Yang, C. G.,Jiang, M..

[18]Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid. Zhu, Hua Ping,Lu, Mai Xin,Gao, Feng Ying,Huang, Zhang Han,Yang, Li Ping,Gui, Jian Fang.

[19]Effect of biofloc technology on growth, digestive enzyme activity, hematology, and immune response of genetically improved farmed tilapia (Oreochromis niloticus). Long, Lina,Yang, Jing,Guan, Chongwu,Wu, Fan,Li, Yuan.

[20]Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus). Yang, Chang Geng,Tian, Juan,Liu, Wei,Wu, Fan,Jiang, Ming,Wen, Hua,Wang, Xian Li.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台