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Analysis of differential gene expression under low-temperature stress in Nile tilapia (Oreochromis niloticus) using digital gene expression

文献类型： 外文期刊

作者： Yang, Changgeng 1 ; Jiang, Ming 1 ; Wen, Hua 1 ; Tian, Juan 1 ; Liu, Wei 1 ; Wu, Fan 1 ; Gou, Gengwu 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodiveis Conservat & Utilizat, Wuhan 430223, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr, Key Lab Freshwater Biodiveis

关键词： Digital gene expression;Tilapia;Low-temperature stress

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

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收录情况： SCI

摘要： Tilapia (Oreochromis niloticus) do not survive well at low temperatures. Therefore, improvement of the low-temperature resistance has become an important issue for aquaculture development of tilapia. The objective of this study was to construct a digital gene expression tag profile to identify genes potentially related to low temperature in tilapia. In this study, tilapia was treated at 30 degrees C to lethal temperature 10 degrees C in decrement of 1 degrees C D-1. Digital gene expression analysis was performed using the Illumina technique to investigate differentially expressed genes in tilapia cultured at different temperatures (30 degrees C, 26 degrees C, 20 degrees C, 16 degrees C, and 10 degrees C). A total of 206,861, 188,082, 185,827, 188,067, and 214,171 distinct tags were obtained by sequencing these five libraries, respectively. Compared with the 30 degrees C library, there were 304, 407, 709, and 772 upregulated genes and 342, 793, 771, and 1466 downregulated genes in 26 degrees C, 20 degrees C, 16 degrees C, and 10 degrees C libraries, respectively. Trend analysis of these differentially expressed genes identified six statistically significant trends. Functional annotation analysis of the differentially expressed genes identified various functions associated with the response to low-temperature stress. When tilapia are subjected to low-temperature stress, expression changes were observed in genes associated with nucleic acid synthesis and metabolism, amino acid metabolism and protein synthesis, lipid and carbohydrate content and types, material transport, apoptosis, and immunity. The differentially expressed genes obtained in this study may provide useful insights to help further understand the effects of low temperature on tilapia. (C) 2015 Elsevier B.V. All rights reserved.

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