文献类型： 外文期刊

作者： Jing, Pu ¹ ; Qian, Bingjun ¹ ; Zhao, Shujuan ¹ ; Qi, Xin ³ ; Ye, Ludan ¹ ; Giusti, M. Monica ⁴ ; Wang, Xingya ² ;

作者机构： 1.Shanghai Jiao Tong Univ, Bor S Luh Food Safety Res Ctr, Res Ctr Food Safety & Nutr, Sch Agr & Biol,Key Lab Urban Agr South, Shanghai 200240, Peoples R China

2.Zhejiang Chinese Med Univ, Coll Pharmaceut Sci, Hangzhou 310053, Zhejiang, Peoples R China

3.Tianjin Acad Agr Sci, Hort Engn Inst, Tianjin 300122, Peoples R China

4.Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA

关键词： Antioxidants;Delphinidin derivatives;HT-29;HCT-116;Glycosylation

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： In this study, we compared the scavenging ROS of anthocyanins from Chinese eggplant var. Niu Jiao Qie and other delphinidin derivatives with different glycosylation patterns in HT-29 and HCT-116 cell lines. The eggplant anthocyanins were isolated and identified using LC-MSn and H-1/C-13 NMR as delphinidin-3-[(4 ''-trans-p-coumaroyl)-rhamnosyl (1 -> 6) glucoside]-5-glucoside, also known as nasunin. Delphinidin derivatives with glycosylation only on C3 (delphinidin-3-glucoside, 3-sambubioside, or 3-rutinoside) exhibited greater effects on ROS reduction as compared to delphinidin derivatives that have glycosylation on C3 and C5 (delphinidin-3,5-diglucoside > delphinidin-3-rutinoside-5-glucoside). Nasunin has glycosylation on C3 and C5 and an acyl group (p-coumaric acid), demonstrated the least effect on ROS reduction. Meanwhile, their ROS reduction activities were consistent with glutathione reductase protein expression levels in HT-29. Although not potent in ROS reduction, nasunin and its deacylated derivatives protected cells from DNA damage in a dose-dependent manner. Taken together, our results suggest that the anthocyanins isolated from Chinese eggplant var. Niu Jiao Qie and other delphinidin have antioxidant activities in colon cancer cells and also protect cells from DNA damage. (C) 2014 Elsevier Ltd. All rights reserved.