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Identification of Type I IFN in Chinese giant salamander (Andrias davidianus) and the response to an iridovirus infection

文献类型： 外文期刊

作者： Chen, Qian 1 ; Ma, Jie 2 ; Fan, Yuding 2 ; Meng, Yan 2 ; Xu, Jin 2 ; Zhou, Yong 2 ; Liu, Wenzhi 2 ; Zeng, Xianhui 2 ; Zeng, 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

关键词： Chinese giant salamander;Andrias davidianus;Type I IFN;Antiviral activity;Innate immunity

期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：4.407； 五年影响因子：4.227 ）

ISSN：

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收录情况： SCI

摘要： The type I IFNs play a major role in the first line of defense against virus infections. In this study, the type I IFN gene designated gsIFN was identified and characterized in the Chinese giant salamander (Andrias davidianus). The genomic DNA of gsIFN contains 5 exons and 4 introns and has a total length of 5622 bp. The full-length cDNA sequence of gsIFN is 1113 bp and encodes a putative protein of 186 amino acids that has a 43% identity to type I IFN of Xenopus tropicalis. The deduced amino acid sequence has the C-terminal CAWE motif, that is mostly conserved in the higher vertebrate type I IFNs. Real-time fluorescence quantitative RT-PCR analysis revealed broad expression of gsIFN in vivo and the highest level expression in blood, kidney and spleen. Additionally, the expression of gsIFN at the mRNA level was significantly induced in peripheral blood leucocytes after stimulation with poly I:C and after infection with the Chinese giant salamander iridovirus (GSIV). A plasmid expressing gsIFN was constructed and transfected into the Chinese giant salamander muscle cell line. Expression of the IFN-inducible gene Mx was up-regulated in the gsIFN-overexpressing cells after GSIV infection. The virus load and titer were significantly reduced compared with that in control cells. Additionally, a lower level of virus major capsid protein synthesis was confirmed by immunofluorescence assay compared to the control cells. These results suggest that the gsIFN gene plays an important role in the antiviral innate immune response. (C) 2015 Elsevier Ltd. All rights reserved.

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