您好,欢迎访问中国水产科学研究院 机构知识库!

Expression Profiling Analysis of the microRNA Response of Cynoglossus semilaevis to Vibrio anguillarum and Other Stimuli

文献类型: 外文期刊

作者: Gong, Guangye 1 ; Sha, Zhenxia 1 ; Chen, Songlin 1 ; Li, Chao 2 ; Yan, Hui 1 ; Chen, Yadong 1 ; Wang, Tianzi 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Yellow Sea Fisheries Res Inst, Minist Agr, Qingdao 266071, Peoples R China

2.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China

3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

关键词: Cynoglossus semilaevis;miRNA;Microarray;Immune;Vibrio anguillarum

期刊名称:MARINE BIOTECHNOLOGY ( 影响因子:3.619; 五年影响因子:3.322 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To investigate the roles of microRNAs (miRNA) of Cynoglossus semilaevis in response to Vibrio anguillarum that were previously identified using high-throughput sequencing, microarray analyses was performed on three small RNA libraries (CG, NOSG, and HOSG) prepared from C. semilaevis immune tissues. In total, of 1279 designed probes, 739 (57.78 %) were detectable. The expression levels of these miRNAs were analyzed using pairwise comparisons among the three libraries, and a total of 99 miRNAs were observed to be significantly differentially expressed. The expression patterns of 10 differentially expressed miRNAs were validated by real-time quantitative PCR (RT-qPCR). In addition, expression of miR-142-5p, miR-223, and miR-181a in response to V. anguillarum at numerous time-points in four tissues, as well as the responses to lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), peptidoglycan (PGN), and red-spotted grouper nervous necrosis virus (RGNNV) in head kidney cells, were studied by qRT-PCR. Taken together, all of the expression profiles showed significant differences compared to the control group; both similarities and differences in the expression responses to the same pathogen were observed. Collectively, these findings highlighted the putative roles for miRNAs in the context of the innate immune response of C. semilaevis exposing to pathogens and that further studies are needed to understand the molecular mechanisms of miRNA regulation in C. semilaevis host-pathogen interactions.

  • 相关文献

[1]Identification and characterization of Cynoglossus semilaevis microRNA response to Vibrio anguillarum infection through high-throughput sequencing. Sha, Zhenxia,Gong, Guangye,Lu, Yang,Wang, Lei,Chen, Songlin,Gong, Guangye,Lu, Yang,Wang, Shaolin,Wang, Qilong.

[2]Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection. Zhang, Xiang,Chen, Songlin,Chen, Yadong,Shao, Changwei,Wang, Qilong,Lu, Yang,Gong, Guangye,Sha, Zhenxia,Zhang, Xiang,Ding, Shaoxiong,Wang, Shaolin,Liu, Yang.

[3]Expression Profile Analysis of miR-221 and miR-222 in Different Tissues and Head Kidney Cells of Cynoglossus semilaevis, Following Pathogen Infection. Yan, Hui,Chen, Yadong,Gong, Guangye,Chen, Xuejie,Wang, Tianzi,Chen, Songlin,Sha, Zhenxia,Chen, Yadong,Chen, Songlin,Sha, Zhenxia,Zhou, Shun,Li, Chao.

[4]Gene cloning and expression analysis of IRF1 in half-smooth tongue sole (Cynoglossus semilaevis). Lu, Yang,Wang, Qilong,Shao, Changwei,Chen, Songlin,Sha, Zhenxia,Lu, Yang,Liu, Yang.

[5]MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis). Du, Min,Chen, Song-lin,Liu, Yang,Yang, Jing-feng,Du, Min,Du, Min,Liu, Yan-hong. 2011

[6]Transcriptome profiling and digital gene expression analysis of Nile tilapia (Oreochromis niloticus) infected by Streptococcus agalactiae. Zhang, Rui,Zhang, Li-li,Ye, Xing,Tian, Yuan-yuan,Sun, Cheng-fei,Lu, Mai-xin,Bai, Jun-jie,Zhang, Rui.

[7]Heterosis of haernolymph analytes of two geographic populations in Chinese shrimp Fenneropenaeus chinensis. Yang, Cuihua,Kong, Jie,Wang, Qingyin,Liu, Qinghui,Tian, Yi,Luo, Kun. 2007

[8]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[9]Ability of Lactobacillus plantarum lipoteichoic acid to inhibit Vibrio anguillarum-induced inflammation and apoptosis in silvery pomfret (Pampus argenteus) intestinal epithelial cells. Gao, Quanxin,Min, Minghua,Zhang, Chenjie,Peng, Shiming,Shi, Zhaohong,Gao, Qian.

[10]RNA-Seq analysis of immune-relevant genes in Lateolabrax japonicus during Vibrio anguillarum infection. Zhao, Chao,Fu, Mingjun,Wang, Chengyang,Qiu, Lihua,Wang, Chengyang,Zhao, Chao,Fu, Mingjun,Wang, Chengyang,Qiu, Lihua,Qiu, Lihua,Jiao, Zongyao.

[11]A farnesoic acid O-methyltransferase (FAMeT) from Exopalaemon carinicauda is responsive to Vibrio anguillarum and WSSV challenge. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun.

[12]Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei,Wang, Yun. 2014

[13]Efficacy of Vibrio anguillarum antigen administered by intraperitoneal injection route in Japanese flounder, Paralichthys olivaceus (Temminck et Schlegel). Li, J,Gao, DM,Wang, Q,Wang, JY,Wang, QY. 2005

[14]Cloning, characterization, and expression of a novel member of proteasomal subunits gene in turbot, Scophthalmus maximus. Zhang Bo,Song Wenping,Zheng Debin,Ma Chao,Xiao Guangxia,Wang Xianli. 2015

[15]Effects of dietary supplementation of A3 alpha-peptidoglycan on innate immune responses and defense activity of Japanese flounder (Paralichthys olivaceus). Zhou, J,Song, XL,Huang, J,Wang, XH. 2006

[16]MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families. Xu, Tian-jun,Chen, Song-lin,Ji, Xiang-shan,Tian, Yong-sheng,Xu, Tian-jun. 2008

[17]Cloning and characterization of type II interleukin-1 receptor cDNA from Japanese flounder (Paralichthys olivaceus). Fan, Yuding,Qi, Jie,Zhong, Qiwang,Zhang, Quanqi,Fan, Yuding,Zeng, Lingbing,Li, Shuo. 2010

[18]MHC class II alpha gene polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Xu, Tian-jun,Chen, Song-lin,Zhang, Yu-xi,Xu, Tian-jun. 2010

[19]Identification and expression analysis of fetuin B (FETUB) in turbot (Scophthalmus maximus L.) mucosal barriers following bacterial challenge. Li, Chao,Gao, Chengbin,Fu, Qiang,Chen, Jinghua,Su, Baofeng,Su, Baofeng.

[20]Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge. Gao, Chengbin,Cai, Xin,Song, Huanhuan,Wang Wenqi,Li, Chao,Su, Baofeng,Su, Baofeng,Zhang, Yu.

作者其他论文 更多>>