Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio)
文献类型: 外文期刊
作者: Tian, Juan 1 ; He, Gen 1 ; Mai, Kangsen 1 ; Liu, Chengdong 1 ;
作者机构: 1.Ocean Univ China, Key Lab Aquaculture Nutr, Minist Agr, Qingdao 266003, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist Agr, Wuhan 430223, Hubei, Peoples R China
关键词: Postprandial starvation;Transporters;Metabolic enzymes;Zebrafish
期刊名称:FISH PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:2.794; 五年影响因子:2.876 )
ISSN:
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收录情况: SCI
摘要: The goal of this study was to systematically evaluate the molecular activities of endocrine-, amino acid and peptide transporters-, and metabolic enzyme-related genes in 35-day-old mixed-sex zebrafish (Danio rerio) after feeding. Zebrafish with initial body weights ranging from 9 to 11 mg were fasted for 384 h in a controlled indoor environment. Fish were sampled at 0, 3, 6, 12, 24, 48, 96, 192, and 384 h after fed. Overall, the present study results show that the regulatory mechanism that insulin-like growth factor I negative feedback regulated growth hormone is conserved in zebrafish, as it is in mammals, but that regulation of growth hormone receptors is highly intricate. Leptin and cholecystokinin are time-dependent negative feedback signals, and neuropeptide Y may be an important positive neuropeptide for food intake in zebrafish. The amino acid/carnitine transporters B-0,B-+ (ATB(0,+)) and broad neutral (0) amino acid transporter 1(B-0 AT1) mRNA levels measured in our study suggest that protein may be utilized during 24-96 h of fasting in zebrafish. Glutamine synthetase mRNA levels were downregulated, and glutamate dehydrogenase, alanine aminotransferase, aspartate transaminase, and trypsin mRNA levels were upregulated after longtime fasting in this study. The mRNA expression levels of fatty acid synthetase decreased significantly (P < 0.05), whereas those of lipoprotein lipase rapidly increased after 96 h of fasting. Fasting activated the expression of glucose synthesis genes when fasting for short periods of time; when fasting is prolonged, the mRNA levels of glucose breakdown enzymes and pentose phosphate shunt genes decreased.
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