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Microspore embryogenesis and plant regeneration in Brussels sprouts (Brassica oleracea L. var. gemmifera)

文献类型： 外文期刊

作者： Zeng, Aisong 1 ; Yan, Yuanyuan 1 ; Yan, Jiyong 2 ; Song, Lixiao 1 ; Gao, Bing 2 ; Li, Jianqi 3 ; Hou, Xilin 1 ; Li, Ying 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Jiangsu, Peoples R China

3.Qingdao Bioivy Crop Sci Co Ltd, Qingdao 2661

关键词： Microspore culture;Embryogenesis;Cytology;Heat and cold treatment;Aminoethoxyvinylglycine;Brussels sprouts

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

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收录情况： SCI

摘要： As a tool in genetic engineering, isolated microspore cultures have the remarkable quality to produce doubled haploids for plant breeding and gene mapping. Protocols were developed for the induction of microspore-derived embryos and generation of doubled haploid plants from isolated microspores of Brussels sprouts. A cytological analysis showed that two modes of Brussels sprouts microspore embryogenesis exist: a direct route via embryos which was the major developmental pathway, and an indirect route via calli. Cold pretreatment (4 degrees C) and aminoethoxyvinylglycine addition were tested to determine whether and how they could improve embryogenesis. Cold pretreatment improved the viability of microspores, and stimulated to produce embryos directly. Similarly, aminoethoxyvinylglycine had a positive effect on the number of embryos produced via improving multinucleate structures to further develop. The combination of cold pretreatment for 24 h and 1 mu M aminoethoxyvinylglycine addition significantly enhanced microspore embryogenesis efficiency, especially with low responsive genotype '1076' for which it was increased by about 82-fold. Subsequently, the use of solid B5 medium with 1% agarose and 2% sucrose (w/v) achieved an efficient rate of plant regeneration, and more than 67% of regenerated plants were spontaneous diploids. (c) 2015 Elsevier B.V. All rights reserved.

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