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Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata

文献类型： 外文期刊

作者： Wan, Pin-Jun 1 ; Fu, Kai-Yun 1 ; Lu, Feng-Gong 1 ; Guo, Wen-Chao 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

3.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

关键词： Leptinotarsa decemlineata;Alanine aminotransferase;RNA interference;Enzyme activity;Flight;Mortality

期刊名称：AMINO ACIDS （ 影响因子：3.52； 五年影响因子：3.6 ）

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收录情况： SCI

摘要： Alanine aminotransferase (ALT) plays important physiological and biochemical roles in insect. In this study, a full-length Ldalt cDNA was cloned from Leptinotarsa decemlineata. It was ubiquitously expressed in the eggs, larvae, pupae and adults. In the adults, Ldalt mRNA was widely distributed in thorax muscles, fat body, midgut, foregut, hindgut, Malpighian tubules, ventral ganglion and epidermis, with the expression levels from the highest to the lowest. Two double-stranded RNAs (dsRNAs) (dsLdalt1 and dsLdalt2) targeting Ldalt were constructed and bacterially expressed. After adults fed on dsLdalt1- and dsLdalt2-immersed foliage for 3 day, Ldalt mRNA abundance was significantly decreased by 79.5 and 71.1 %, and ALT activities were significantly reduced by 64.5 and 67.6 %, respectively. Moreover, silencing Ldalt affected free amino acid contents. Lysine was decreased by 100.0 and 100.0 %, and arginine was reduced by 87.5 and 89.4 %, respectively, in the hemolymph from dsLdalt1- and dsLdalt2-ingested beetles, compared with control ones. In contrast, proline was increased by 88.7 and 96.4 %. Furthermore, ingestion of dsLdalt1 and dsLdalt2 significantly decreased flight speed, shortened flight duration time and flight distance. In addition, knocking down Ldalt significantly increased adult mortality. These data imply that LdALT plays important roles in amino acid metabolism and in flight in L. decemlineata.

  • 相关文献

[1]Knocking down a putative Delta(1)-pyrroline-5-carboxylate dehydrogenase gene by RNA interference inhibits flight and causes adult lethality in the Colorado potato beetle Leptinotarsa decemlineata (Say). Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Wang, Xin-Xin,Li, Guo-Qing,Wan, Pin-Jun,Guo, Wen-Chao.

[2]A putative Delta(1)-pyrroline-5-carboxylate synthetase involved in the biosynthesis of proline and arginine in Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[3]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

[4]RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Mu, Li-Li,Chen, Xu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[5]Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Fu, Kai-Yun,Li, Qian,Zhou, Li-Tao,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[6]Identification of ten mevalonate enzyme-encoding genes and their expression in response to juvenile hormone levels in Leptinotarsa decemlineata (Say). Li, Qian,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[7]CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[8]Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle. Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao.

[9]The subunit gene Ld alpha 1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata. Qu, Yang,Chen, Jinhua,Li, Chenge,Wang, Qiang,Han, Zhaojun,Jiang, Weihua,Guo, Wenchao.

[10]Physiological roles of trehalose in Leptinotarsa larvae revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes. Shi, Ji-Feng,Xu, Qing-Yu,Sun, Qiang-Kun,Meng, Qing-Wei,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[11]Novaluron ingestion causes larval lethality and inhibits chitin content in Leptinotarsa decemlineata fourth-instar larvae. Xu, Qing-Yu,Meng, Qing-Wei,Shi, Ji-Feng,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao. 2017

[12]Transcription changes of a putative trehalose-6-phosphate synthase gene in response to hormone stimulation in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Xu, Qing-Yu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[13]RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Zhou, Li-Tao,Jia, Shuang,Wan, Pin-Jun,Kong, Ye,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[14]Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say). Shi, Xiao-qin,Xiong, Man-Hui,Jiang, Wei-Hua,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun. 2012

[15]Effect of Temperature on the Occurrence and Distribution of Colorado Potato Beetle (Coleoptera: Chrysomelidae) in China. Liu, Huai,Wang, Jin-Jun,Li, Chao,Cheng, Deng-Fa,Zhang, Yun-Hui,Sun, Jin-Rui,Huang, Fangneng,Guo, Wen-Chao. 2014

[16]Receptor tyrosine kinase genes respond transcriptionally to sublethal doses of five insecticides by a mode-of-action independent way in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Zhu, Tao-Tao,Li, Guo-Qing,Guo, Wen-Chao. 2016

[17]The basic helix-loop-helix transcription factors in the Colorado potato beetle Leptinotarsa decemlineata. Fu, Kai-Yun,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2015

[18]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

[19]Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference. Guo, Wen-Chao,Liu, Xin-Ping,Fu, Kai-Yun,Shi, Ji-Feng,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[20]Transcription response of three putative trehalase genes to hormonal stimulation in the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Shi, Ji-Feng,Sun, Qiang-Kun,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

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