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Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference

文献类型： 外文期刊

作者： Guo, Wen-Chao 1 ; Liu, Xin-Ping 1 ; Fu, Kai-Yun 1 ; Shi, Ji-Feng 1 ; Lu, Feng-Gong 1 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Educ Minist, Key Lab Integrated Management Crop Dis & Pests, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

关键词： Leptinotarsa decemlineata;HR3;20-Hydroxyecdysone;Pupation

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

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收录情况： SCI

摘要： Our previous results revealed that RNA interference-aided knockdown of Leptinotarsa decemlineata FTZ-F1 (LdFTZ-F1) reduced 20E titer, and impaired pupation. In this study, we characterized a putative LdHR3 gene, an early-late 20E-response gene upstream of LdFTZ-F1. Within the first, second and third larval instars, three expression peaks of LdHR3 occurred just before the molt. In the fourth (final) larval instar 80 h after ecdysis and prepupal stage 3 days after burying into soil, two LdHR3 peaks occurred. The LdHR3 expression peaks coincide with the peaks of circulating 20E level. In vitro midgut culture and in vivo bioassay revealed that 20E and an ecdysteroid agonist halofenozide (Hal) enhanced LdHR3 expression in the final larval instars. Conversely, a decrease in 20E by feeding a double-stranded RNA (dsRNA) against an ecdysteroidogenesis gene Ldshd repressed the expression. Moreover, Hal rescued the transcript levels in the Ldshd-silenced larvae. Thus, 20E peaks activate the expression of LdHR3. Furthermore, ingesting dsRNA against LdHR3 successfully knocked down the target gene, and impaired pupation. Finally, knockdown of LdHR3 upregulated the transcription of three ecdysteroidogenesis genes (Ldphm, Lddib and Ldshd), increased 20E titer, and activated the expression of two 20E-response genes (LdEcR and LdFTZ-F1). Thus, LdHR3 functions in regulation of pupation in the Colorado potato beetle. (C) 2015 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]Involvement of FTZ-F1 in the regulation of pupation in Leptinotarsa decemlineata (Say). Liu, Xin-Ping,Fu, Kai-Yun,Lu, Feng-Gong,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[2]Novaluron ingestion causes larval lethality and inhibits chitin content in Leptinotarsa decemlineata fourth-instar larvae. Xu, Qing-Yu,Meng, Qing-Wei,Shi, Ji-Feng,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao. 2017

[3]Transcription changes of a putative trehalose-6-phosphate synthase gene in response to hormone stimulation in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Xu, Qing-Yu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[4]Transcription response of three putative trehalase genes to hormonal stimulation in the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Shi, Ji-Feng,Sun, Qiang-Kun,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[5]Nuclear receptor ecdysone-induced protein 75 is required for larval-pupal metamorphosis in the Colorado potato beetle Leptinotarsa decemlineata (Say). Guo, W-C.,Liu, X-P.,Fu, K-Y.,Shi, J-F.,Lu, F-G.,Li, G-Q.,Guo, W-C..

[6]Knockdown of a putative insulin-like peptide gene LdILP2 in Leptinotarsa decemlineata by RNA interference impairs pupation and adult emergence. Fu, Kai-Yun,Zhu, Tao-Tao,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun.

[7]Leptinotarsa cap 'n' collar isoform C/Kelch-like ECH associated protein 1 signaling is critical for the regulation of ecdysteroidogenesis in the larvae. Sun, Qiang-Kun,Meng, Qing-Wei,Xu, Qing-Yu,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao.

[8]IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY). Shi, Ji-Feng,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[9]RNA interference suppression of the receptor tyrosine kinase Torso gene impaired pupation and adult emergence in Leptinotarsa decemlineata. Zhu, Tao-Tao,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[10]RNA interference of a putative S-adenosyl-L-homocysteine hydrolase gene affects larval performance in Leptinotarsa decemlineata (Say). Zhou, Li-Tao,Jia, Shuang,Wan, Pin-Jun,Kong, Ye,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[11]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

[12]RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Mu, Li-Li,Chen, Xu,Li, Guo-Qing,Guo, Wen-Chao. 2016

[13]Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say). Shi, Xiao-qin,Xiong, Man-Hui,Jiang, Wei-Hua,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun. 2012

[14]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[15]A putative Delta(1)-pyrroline-5-carboxylate synthetase involved in the biosynthesis of proline and arginine in Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[16]Effect of Temperature on the Occurrence and Distribution of Colorado Potato Beetle (Coleoptera: Chrysomelidae) in China. Liu, Huai,Wang, Jin-Jun,Li, Chao,Cheng, Deng-Fa,Zhang, Yun-Hui,Sun, Jin-Rui,Huang, Fangneng,Guo, Wen-Chao. 2014

[17]Knocking down a putative Delta(1)-pyrroline-5-carboxylate dehydrogenase gene by RNA interference inhibits flight and causes adult lethality in the Colorado potato beetle Leptinotarsa decemlineata (Say). Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Wang, Xin-Xin,Li, Guo-Qing,Wan, Pin-Jun,Guo, Wen-Chao.

[18]Receptor tyrosine kinase genes respond transcriptionally to sublethal doses of five insecticides by a mode-of-action independent way in Leptinotarsa decemlineata (Say). Shi, Ji-Feng,Zhu, Tao-Tao,Li, Guo-Qing,Guo, Wen-Chao. 2016

[19]The basic helix-loop-helix transcription factors in the Colorado potato beetle Leptinotarsa decemlineata. Fu, Kai-Yun,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2015

[20]Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Fu, Kai-Yun,Li, Qian,Zhou, Li-Tao,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

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