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Calreticulin is required for responding to stress, foraging, and fertility in the white-tip nematode, Aphelenchoides besseyi

文献类型： 外文期刊

作者： Feng, Hui 1 ; Wei, Lihui 1 ; Chen, Huaigu 1 ; Zhou, Yijun 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词： Aphelenchoides besseyi;Calreticulin;cDNA cloning;RNAi

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

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收录情况： SCI

摘要： Calreticulin (CRT) regulates a wide array of cellular responses in physiological and pathological processes. A full-length cDNA-encoding CRT protein, namely AbCRT-1, was isolated from Aphelenchoides besseyi, an ectoparasitic plant nematode and the agent of white tip disease of rice. The deduced amino acid sequence of AbCRT-1 was highly homologous with other nematode CRTs, and showed the closest evolutionary relationship with BxCRT-1. In-situ hybridization showed that AbCRT-1 is specifically located in the oesophageal gland and gonads of A. besseyi, suggesting its potential role in parasitism and reproduction. Quantity real-time PCR analysis showed that AbCRT-1 is highly expressed in female nematodes but poorly expressed in eggs, juveniles, and male nematodes. Exposing the nematode to relatively low osmotic stress promotes the transcription of AbCRT-1 whereas extreme desiccation suppresses the transcription significantly. Nematodes in which AbCRT-1 mRNA level had been knocked down by soaking them in AbCRT-1 dsRNA solution distributed randomly and did not aggregate temporally, with a decreased capacity of food discernment. Thus the affected nematodes were markedly less fecund. These results demonstrate that AbCRT-1 is required in A. besseyi for responding to stress, foraging, and fertility. (C) 2015 Elsevier Inc. All rights reserved.

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