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A Specific and High-Throughput Fluorescence Polarization Immunoassay for Surveillance Screening of Clinafloxacin in Milk

文献类型： 外文期刊

作者： Chen, Jiahong 1 ; Lv, Shuwei 1 ; Wang, Qiang; Xu, Zhenlin 1 ; Yang, Jinyi 1 ; Shen, Yudong 1 ; Wang, Hong 2 ; Sun, Yua 1 ;

作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Storag, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Storag, Guangzhou 510642, Guangdon

关键词： Clinafloxacin;Homogeneous;Fluorescence polarization immunoassay;Milk

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

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收录情况： SCI

摘要： In this study, antibody specific to clinafloxacin (CLI) was generated for the first time, and a rapid and specific surveillance screening method, fluorescence polarization immunoassay (FPIA), was developed for the detection of CLI residue in milk. The developed FPIA showed a working range from 0.8 to 787.3 mu g/L with the IC50 of 25.6 mu g/L. The cross-reactivity to other fluoroquinolone drugs was negligible, which demonstrated high specificity. Good recoveries, ranged from 97.9 to 112.7 %, in spiked milk samples were obtained. The proposed FPIA was also validated by high performance liquid chromatography as a reference method, and the two methods yielded concordant results. The investigation indicated that the proposed FPIA is suitable for surveillance screening purposes to detect CLI residue in milk.

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[1]Heterologous strategy enhancing the sensitivity of the fluorescence polarization immunoassay of clinafloxacin in goat milk. Shanin, Ilya A.,Eremin, Sergei A.,Chen, Jiahong,Lv, Shuwei,Xu, Zhenlin,Sun, Yuanming,Wu, Qing,Lei, Hongtao,Shanin, Ilya A.,Eremin, Sergei A.,Wang, Qiang,Mao, Chuanbin.

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