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Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus

文献类型： 外文期刊

作者： Zheng, Y. 1 ; Peng, L. M. 1 ; You, F. 1 ; Zou, Y. X. 1 ; Zhang, P. J. 1 ; Chen, S. L. 3 ;

作者机构： 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China

2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao, Shandong, Peoples R China

关键词： astrocyte;brain-cell line;epithelioid-like;karyotype;transfection

期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）

ISSN：

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收录情况： SCI

摘要： A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.

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