Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)
文献类型： 外文期刊
作者： Chen, Wei 1 ; Li, Dunsong 2 ; Zhang, Min 1 ; Zhao, Yunlong 1 ; Wu, Wenjing 1 ; Zhang, Guren 1 ;
作者机构： 1.Sun Yat Sen Univ, Inst Entomol, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Plant Protect, Guangzhou 510640, Guangdong, Peoples R China
关键词： Neoseiulus cucumeris (Oudemans);Heat shock protein;Thermal stress;Developmental stage
期刊名称：EXPERIMENTAL AND APPLIED ACAROLOGY （ 影响因子：2.132； 五年影响因子：2.196 ）
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收录情况： SCI
摘要： In order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90, NcHsp75, NcHsp70, NcHsp60, and NcHsp40. These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90, NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35-45 degrees C); however, a significant down-regulation was observed after 3 h heat exposure at 35 degrees C and 3 h recovery at 25 degrees C. Cold shock treatment (-5 to 15 degrees C) for 1 h did not acute elicit changes in the expression levels
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