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In-depth transcriptome analysis of Coilia ectenes, an important fish resource in the Yangtze River: de novo assembly, gene annotation

文献类型： 外文期刊

作者： Shen, Huaishun 1 ; Gu, Ruobo 1 ; Xu, Gangchun 1 ; Xu, Pao 1 ; Nie, Zijuan 1 ; Hu, Yacheng 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China

2.Nanjing Agr Univ, Wuxi Fisheries Coll, Nanjing 210095, Jiangsu, Peoples R China

关键词： Coilia ectenes;Transcriptome;Transcript;Next generation sequencing

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Coilia ectenes is an important teleost species in the Yangtze River and a model organism that can be used to study the protection of fish resources. In this report, we performed de nova transcriptome sequencing of ten cDNA libraries from the brain, gill, heart, intestine, kidney, liver, muscle, stomach, ovary, and testis tissues. A total of 352 million raw reads of 100 base pairs were generated, and 130,113 transcripts, corresponding to 65,350 non-redundant transcripts, with a mean length of 1520 bp, were assembled. BLASTx-based gene annotation (E-value < 1 x 10(-5)) allowed the identification of 73,900 transcripts against at least one of four databases, including the NCBI non-redundant database, the GO database, the COG database, and the KEGG database. Our study provides a valuable resource for C. ectenes genomic and transcriptomic data that will facilitate future functional studies of C ectenes. (C) 2015 Published by Elsevier B.V.

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