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IGF-I slightly improves nuclear maturation and cleavage rate of bovine oocytes exposed to acute heat shock in vitro

文献类型： 外文期刊

作者： Qi Meiyu 1 ; Liu, Di 1 ; Roth, Zvi 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Harbin, Peoples R China; Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel

关键词： Bovine;Cleavage rate;Heat shock;IGF-I;Oocyte maturation

期刊名称：ZYGOTE （ 影响因子：1.442； 五年影响因子：1.536 ）

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收录情况： SCI

摘要： An in vitro model of embryo production was used to examine the effects of insulin-like growth factor (IGF)-I on maturation and developmental competence of oocytes exposed to heat shock. Cumulus-oocyte complexes were matured at 38.5 degrees C or exposed to acute heat shock (HS; 41.5 degrees C), with or without 100 ng/ml IGF-I, for 22 h through in vitro maturation. The experimental groups were control (C), C + IGF-I, HS, and HS + IGF-I. Oocytes were fertilized at the end of maturation, and the proportion of cleaved embryos was recorded 44 h later. HS during maturation increased the proportion of TUNEL-positive oocytes (P < 0.05). HS did not have any effect on cortical granule translocation but impaired resumption of meiosis, expressed as a decreased proportion of oocytes with nuclei in metaphase I (P < 0.05) and metaphase II (MII; P < 0.05). HS decreased the proportion of oocytes that cleaved (P < 0.05), in particular those oocytes that further developed to 4-cell-stage embryos (P < 0.05). IGF-I alleviated, to some extent, the deleterious effects of HS on the oocytes as reflected by a reduced proportion of TUNEL-positive oocytes (P < 0.03). While not significant, IGF-I tended to increase the proportion of MII-stage oocytes (P < 0.08) and 4-cell-stage cleaved embryos (P < 0.06). Further examination is required to explore whether IGF-I also affects the developmental competence of oocytes exposed to HS.

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[1]Embryotropic actions of follistatin: paracrine and autocrine mediators of oocyte competence and embryo developmental progression. Rajput, Sandeep K.,Lee, KyungBon,Guo Zhenhua,Folger, Joseph K.,Smith, George W.,Guo Zhenhua,Di, Liu. 2014

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