Production of Biological Control Agent Bacillus subtilis B579 by Solid-State Fermentation using Agricultural Residues
文献类型： 外文期刊
作者： Tian, Tao 1 ; Sun, Bing Bing 1 ; Sun, Shu Qin 1 ; Yang, Xiu-Rong 1 ; Li, Wei 1 ; Ma, Ping 2 ; Zhang, Li-Qun 3 ;
作者机构： 1.Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin 300381, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Hebei, Peoples R China
3.China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China
关键词： Agricultural residues;Bacillus subtilis B579;Biological Control agent;Design-Expert;Solid-State Fermentation
期刊名称：JOURNAL OF PURE AND APPLIED MICROBIOLOGY （ 影响因子：0.073； ）
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收录情况： SCI
摘要： Three different agricultural residues were evaluated as basic substrates for the production of biological control agent (BCA) Bacillus subtilis strain B579, by solid-state fermentation (SSF). Among them, wheat bran (WB) was shown to be the most suitable substrate, and an initial moisture content of 57% was determined to be optimal for SSF of strain B579. Moreover, employing the Plackett-Burman and Box-Benhnken experimental design models, nine different supplements were investigated to maximize the cell yield. Results demonstrated that a relatively maximal cell yield of 2.12+/-0.03x10(11) CFU/g was achievable utilizing WB as the basic medium supplemented with beef extract (21.3 g kg(-1)), cornmeal (42.6 g kg(-1)), and soluble starch (23.8 g kg(-1)).
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