Novel Glyphosate-Resistant aroA Gene from Paracoccus denitrificans in Transgenic Arabidopsis
文献类型： 外文期刊
作者： Tian, Y. -S. 1 ; Yao, Q. -H. 2 ; Xing, X. -J. 4 ; Peng, R. -H. 2 ; Xu, J. 2 ; Xiong, A. -S. 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
3.Shanghai Ruifeng Agr Sci & Technol Co Ltd, Shanghai 201106, Peoples R China
4.Shanxi Agr Univ, Coll Hort, Taigu 030801, Peoples R China
关键词： 5-enolpyruvylshikimate-3-phosphate synthase;Paracoccus denitrificans;glyphosate;transgenic Arabidopsis
期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY （ 影响因子：0.886； 五年影响因子：0.956 ）
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年卷期：
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收录情况： SCI
摘要： To date, only aroA variants derived from Agrobacterium tumefaciens CP4 have been used to generate the commercial glyphosate-resistant crops currently available in the market. Therefore, it is of interest to increase aroA gene diversity and seek new glyphosate-tolerant genes for developing glyphosate-tolerant crops. In this study, a novel aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase from Paracoccus denitrificans was isolated using the genomic library construction and complementary screening. Furthermore, the transgenic Arabidopsis plants with the aroA gene from P. denitrificans were obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
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