Heterologous Extracellular Expression and Initial Characterization of the Peroxisomal Catalase from the Methylotrophic Yeast Hansenula polymorpha in Pichia pastoris
文献类型： 外文期刊
作者： Tian, Y. -S. 1 ; Xu, H. 1 ; Xu, J. 1 ; Peng, R. -H. 1 ; Yao, Q. -H. 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
期刊名称：APPLIED BIOCHEMISTRY AND MICROBIOLOGY （ 影响因子：0.886； 五年影响因子：0.956 ）
ISSN： 0003-6838
年卷期： 2013 年 49 卷 5 期
页码：
收录情况： SCI
摘要： Catalase is well known to eliminate H2O2 in cells and reduces the toxicity of peroxide compounds. A catalase gene HpCAT1 of methylotrophic yeast Hansenula polymorpha without the part coding the native signal peptide was cloned into expression vector pYM3165 and then integrated into genome of Pichia pastoris GS115 by electroporation. The result of the enzyme activity assay and SDS-PAGE demonstrated that the recombinant protein (HpCAT1) of H. polymorpha was extracellularly expressed in P. pastoris. The expressed catalase was recovered from the culture supernatant of P. pastoris GS115 and purified by (NH4)(2)SO4 fractionation and Ni-NTA affinity chromatography. The main biochemical properties of the recombinant protein HpCAT1, such as thermodependence and thermostability, pH optimum and pH stability, as well as the effect of metal ions and chemicals, were characterized. With H2O2 as the substrate, HpCAT1 displayed pH and temperature optima of similar to 2.6 and 45A degrees C, respectively. The recombinant HpCAT1 activity was inhibited by 1 mM Hg2+ and Cu2+, but was highly enhanced by 1.0 mM Fe2+.
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