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Virulence, Host-Selective Toxin Production, and Development of Three Cochliobolus Phytopathogens Lacking the Sfp-Type 4 '-Phosphopantetheinyl Transferase Ppt1

文献类型： 外文期刊

作者： Zainudin, Nur Ain Izzati Mohd 1 ; Condon, Bradford 1 ; De Bruyne, Lieselotte 3 ; Van Poucke, Christof 4 ; Bi, Qi 1 ;

作者机构： 1.Cornell Univ, Sch Integrat Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA

2.Univ Putra Malaysia, Fac Sci, Dept Biol, Serdang 43400, Selangor, Malaysia

3.Univ Ghent, Fac Biosci Engn, Lab Phytopathol, Dept Crop Protect, B-9000 Ghent, Belgium

4.Univ Ghent, Fac Pharmaceut Sci, Dept Bioanal, Lab Food Anal, B-9000 Ghent, Belgium

5.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

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收录情况： SCI

摘要： The Sfp-type 4'-phosphopantetheinyl transferase Ppt1 is required for activation of nonribosomal peptide synthetases, including alpha-aminoadipate reductase (AAR) for lysine biosynthesis and polyketide synthases, enzymes that biosynthesize peptide and polyketide secondary metabolites, respectively. Deletion of the PPT1 gene, from the maize pathogen Cochliobolus heterostrophus and the rice pathogen Cochliobolus miyabeanus, yielded strains that were significantly reduced in virulence to their hosts. In addition, ppt1 mutants of C. heterostrophus race T and Cochliobolus victoriae were unable to biosynthesize the host-selective toxins (HST) T-toxin and victorin, respectively, as judged by bioassays. Interestingly, ppt1 mutants of C. miyabeanus were shown to produce tenfold higher levels of the sesterterpene-type non-HST ophiobolin A, as compared with the wild-type strain. The ppt1 strains of all species were also reduced in tolerance to oxidative stress and iron depletion; both phenotypes are associated with inability to produce extracellular siderophores biosynthesized by the nonribosomal peptide synthetase Nps6. Colony surfaces were hydrophilic, a trait previously associated with absence of C. heterostrophus Nps4. Mutants were decreased in asexual sporulation and C. heterostrophus strains were female-sterile in sexual crosses; the latter phenotype was observed previously with mutants lacking Nps2, which produces an intracellular siderophore. As expected, mutants were albino, since they cannot produce the polyketide melanin and were auxotrophic for lysine because they lack an AAR.

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